Back to Search
Start Over
Host Transcriptomic Response Following Administration of Rotavirus Vaccine in Infants’ Mimics Wild Type Infection
- Source :
- Frontiers in Immunology, Vol 11 (2021), Frontiers in Immunology
- Publication Year :
- 2021
- Publisher :
- Frontiers Media S.A., 2021.
-
Abstract
- BackgroundRotavirus (RV) is an enteric pathogen that has devastating impact on childhood morbidity and mortality worldwide. The immunologic mechanism underlying the protection achieved after RV vaccination is not yet fully understood.MethodsWe compared the transcriptome of children affected by community-acquired RV infection and children immunized with a live attenuated RV vaccine (RotaTeq®).ResultsRV vaccination mimics the wild type infection causing similar changes in children’s transcriptome, including transcripts associated with cell cycle, diarrhea, nausea, vomiting, intussusception, and abnormal morphology of midgut. A machine learning approach allowed to detect a combination of nine-transcripts that differentiates vaccinated from convalescent-naturally infected children (AUC: 90%; 95%CI: 70–100) and distinguishes between acute-infected and healthy control children (in both cases, AUC: 100%; 95%CI: 100–100). We identified a miRNA hsa-mir-149 that seems to play a role in the host defense against viral pathogens and may have an antiviral role.DiscussionOur findings might shed further light in the understanding of RV infection, its functional link to intussusception causes, as well as guide development of antiviral treatments and safer and more effective vaccines. The nine-transcript signature may constitute a marker of vaccine protection and helps to differentiate vaccinated from naturally infected or susceptible children.
- Subjects :
- 0301 basic medicine
lcsh:Immunologic diseases. Allergy
Immunology
RNA-Seq
Vaccines, Attenuated
medicine.disease_cause
Rotavirus Infections
Machine Learning
Transcriptome
03 medical and health sciences
transcriptomics
0302 clinical medicine
1108 Medical Microbiology
Rotavirus
medicine
Humans
Immunology and Allergy
030212 general & internal medicine
Child
Disease Resistance
Original Research
miRNA
intussusception
Sequence Analysis, RNA
business.industry
Rotavirus Vaccines
Wild type
Infant
biomarkers
vaccination
Rotavirus vaccine
Community-Acquired Infections
Vaccination
MicroRNAs
Diarrhea
030104 developmental biology
rotavirus
1107 Immunology
Vomiting
medicine.symptom
RNA-seq
business
lcsh:RC581-607
Subjects
Details
- Language :
- English
- ISSN :
- 16643224
- Volume :
- 11
- Database :
- OpenAIRE
- Journal :
- Frontiers in Immunology
- Accession number :
- edsair.doi.dedup.....e3f8c1dbadd6b479a3fd35a9c96c882d