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Valproic acid modulates platelet and coagulation function ex vivo
- Source :
- Blood Coagulation & Fibrinolysis. 28:479-484
- Publication Year :
- 2017
- Publisher :
- Ovid Technologies (Wolters Kluwer Health), 2017.
-
Abstract
- Trauma-induced coagulopathy is associated with adverse patient outcome. Animal models demonstrate that histone deacetylase inhibitors, such as valproic acid (VPA), improve survival following injury. While in-vivo data suggest that improved survival may in part be because of an attenuation of coagulopathy, it remains unknown whether this is a direct effect of the drug, or the establishment of an overall prosurvival phenotype. We thus conducted an ex-vivo experiment to determine if VPA has an effect on coagulation and platelet function. Ten swine were subjected to traumatic brain injury (TBI) and hemorrhagic shock (HS). Blood samples were drawn prior to TBI+HS insult (Healthy group) and 2 h following TBI+HS (Shock group). Samples were incubated with VPA or vehicle controls for 1 h. Platelet aggregation was analyzed via impedance aggregometry and coagulation was measured using thromboelastography. Addition of VPA to the healthy blood did not affect platelet aggregation or coagulation parameters. In shock blood, incubation with VPA significantly reduced collagen-(P = 0.050), arachidonic acid-(P = 0.005), and adenosine diphosphate-(P = 0.023) induced platelet aggregation. VPA also significantly increased the clot strength (P = 0.002) and clot formation rate (P = 0.011). This is the first study to investigate the effect of VPA on platelet function ex vivo. Our results suggest that VPA has no effect on normal blood, but it decreases platelet activation and improves clot dynamics (strength and rate of formation) in blood from shocked animals. This suggests that VPA is capable of exerting a selective platelet sparing effect while enhancing the clot integrity.
- Subjects :
- Blood Platelets
Platelet Aggregation
Swine
Shock, Hemorrhagic
030204 cardiovascular system & hematology
Pharmacology
03 medical and health sciences
0302 clinical medicine
medicine
Coagulopathy
Animals
Platelet
Platelet activation
Blood Coagulation
medicine.diagnostic_test
business.industry
Valproic Acid
Thrombosis
030208 emergency & critical care medicine
Hematology
General Medicine
medicine.disease
Thromboelastography
Coagulation
Brain Injuries
Shock (circulatory)
lipids (amino acids, peptides, and proteins)
medicine.symptom
business
Ex vivo
Subjects
Details
- ISSN :
- 09575235
- Volume :
- 28
- Database :
- OpenAIRE
- Journal :
- Blood Coagulation & Fibrinolysis
- Accession number :
- edsair.doi.dedup.....e3f4d1c317e941af7e67054a4889e4d5