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Field-based dynamic light scattering microscopy: theory and numerical analysis

Authors :: Chulmin Joo
Johannes F. de Boer
Biophotonics and Medical Imaging
Neuroscience Campus Amsterdam - Brain Imaging Technology
LaserLaB - Biophotonics and Microscopy
Source :: Joo, C & de Boer, J F 2013, ' Field-based dynamic light scattering microscopy: theory and numerical analysis ', Applied Optics, vol. 52, no. 1, pp. 7618-7628 . https://doi.org/10.1364/AO.52.007618, Applied Optics, 52(1), 7618-7628. The Optical Society
Publication Year :: 2013
Abstract: We present a theoretical framework for field-based dynamic light scattering microscopy based on a spectral-domain optical coherence phase microscopy (SD-OCPM) platform. SD-OCPM is an interferometric microscope capable of quantitative measurement of amplitude and phase of scattered light with high phase stability. Field-based dynamic light scattering (F-DLS) analysis allows for direct evaluation of complex-valued field autocorrelation function and measurement of localized diffusive and directional dynamic properties of biological and material samples with high spatial resolution. In order to gain insight into the information provided by F-DLS microscopy, theoretical and numerical analyses are performed to evaluate the effect of numerical aperture of the imaging optics.We demonstrate that sharp focusing of fields affects the measured diffusive and transport velocity, which leads to smaller values for the dynamic properties in the sample. An approach for accurately determining the dynamic properties of the samples is discussed. © 2013 Optical Society of America.