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Evidence for biological effects of exogenous LPA on rat primary afferent and spinal cord neurons
- Source :
- Brain Research. 1022:205-213
- Publication Year :
- 2004
- Publisher :
- Elsevier BV, 2004.
-
Abstract
- There is growing behavioural evidence that the phospholipid growth factor lysophosphatidic acid (LPA) modulates nociceptive responses in vivo. The present study investigated further the effects of LPA on peripheral nociceptive processing. Effects of intraplantar injection of LPA on ongoing and peripheral mechanically evoked responses of spinal neurons were studied in vivo. In addition, LPA-evoked responses of adult rat dorsal root ganglion (DRG) neurons were studied with calcium imaging. To determine whether LPA may also act at the level of the spinal cord, LPA receptor G-protein coupling in lumbar spinal cord sections was studied with in vitro autoradiography of guanylyl 5'-[g-[(35)S]thio]triphosphate ([(35)S]GTPgammaS) binding. Intraplantar injection of LPA (5 microg/5 microl) significantly increased the duration (P
- Subjects :
- Male
Narcotics
medicine.medical_specialty
Central nervous system
Action Potentials
In Vitro Techniques
Statistics, Nonparametric
Potassium Chloride
Rats, Sprague-Dawley
Calcium imaging
Dorsal root ganglion
Ganglia, Spinal
Internal medicine
Sulfur Isotopes
Reaction Time
medicine
Animals
Drug Interactions
Neurons, Afferent
Molecular Biology
Pain Measurement
Afferent Pathways
Analysis of Variance
Dose-Response Relationship, Drug
Morphine
Chemistry
General Neuroscience
Spinal cord
Rats
Lumbar Spinal Cord
Endocrinology
medicine.anatomical_structure
Nociception
Spinal Cord
nervous system
Guanosine 5'-O-(3-Thiotriphosphate)
Nociceptor
Autoradiography
Calcium
lipids (amino acids, peptides, and proteins)
Neurology (clinical)
Neuron
Lysophospholipids
biological phenomena, cell phenomena, and immunity
Neuroscience
Developmental Biology
Subjects
Details
- ISSN :
- 00068993
- Volume :
- 1022
- Database :
- OpenAIRE
- Journal :
- Brain Research
- Accession number :
- edsair.doi.dedup.....e3c6ff3c9a2f33f254c6f091b8fac135