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Epigallocatechin-3-O-gallate Inhibits Fibroblast Contraction of Floating Collagen Gel: Interaction between Epigallocatechin-3-O-gallate and Platelet Derived Growth Factor
- Source :
- Bioscience, Biotechnology, and Biochemistry. 68:1817-1820
- Publication Year :
- 2004
- Publisher :
- Informa UK Limited, 2004.
-
Abstract
- The effects of (-)-epigallocatechin gallate (EGCG) on the contraction of floating collagen gel by fibroblasts were investigated. EGCG inhibited collagen gel contraction dose-dependently. On the basis of the fact that platelet-derived growth factor (PDGF) is one of the serum components with stimulatory activity in collagen gel contraction, we examined the possibility that interaction between EGCG and PDGF may be involved in this inhibition mechanism. We confirmed this by recombinant PDGF-BB in the present system and we found that EGCG inhibited PDGF-stimulated collagen gel contraction. The results of affinity chromatography indicated that PDGF was bound by EGCG immobilized on agarose gel as detected by enzyme-linked immunoassay and Western blotting. These findings suggest that binding of EGCG to PDGF is at least partly involved in the mechanism of inhibition of collagen gel contraction by EGCG.
- Subjects :
- Platelet-derived growth factor
medicine.medical_treatment
Epigallocatechin gallate
complex mixtures
Applied Microbiology and Biotechnology
Biochemistry
Catechin
Analytical Chemistry
chemistry.chemical_compound
medicine
Humans
heterocyclic compounds
Fibroblast
Molecular Biology
Cells, Cultured
Platelet-Derived Growth Factor
biology
Growth factor
Organic Chemistry
food and beverages
General Medicine
Gallate
Fibroblasts
Molecular biology
Blot
medicine.anatomical_structure
chemistry
biology.protein
Agarose
Collagen
sense organs
Gels
Platelet-derived growth factor receptor
Biotechnology
Subjects
Details
- ISSN :
- 13476947 and 09168451
- Volume :
- 68
- Database :
- OpenAIRE
- Journal :
- Bioscience, Biotechnology, and Biochemistry
- Accession number :
- edsair.doi.dedup.....e3b5a9046cd5030f2e597a1f52c4b9a7