Back to Search
Start Over
Shear stress inhibits adhesion molecule expression in vascular endothelial cells induced by coculture with smooth muscle cells
- Source :
- Blood. 101:2667-2674
- Publication Year :
- 2003
- Publisher :
- American Society of Hematology, 2003.
-
Abstract
- Vascular endothelial cells (ECs), which exist in close proximity to vascular smooth muscle cells (SMCs), are constantly subjected to blood flow–induced shear stress. Although the effect of shear stress on endothelial biology has been extensively studied, the influence of SMCs on endothelial response to shear stress remains largely unexplored. We examined the potential role of SMCs in regulating the shear stress–induced gene expression in ECs, using a parallel-plate coculture flow system in which these 2 types of cells were separated by a porous membrane. In this coculture system, SMCs tended to orient perpendicularly to the flow direction, whereas the ECs were elongated and aligned with the flow direction. Under static conditions, coculture with SMCs induced EC gene expression of intercellular adhesion molecule-1 (ICAM-1), vascular adhesion molecule-1 (VCAM-1), and E-selectin, while attenuating EC gene expression of endothelial nitric oxide synthase (eNOS). Shear stress significantly inhibited SMC-induced adhesion molecule gene expression. These EC responses under static and shear conditions were not observed in the absence of close communication between ECs and SMCs, and they were also not observed when ECs were cocultured with fibroblasts instead of SMCs. Our findings indicate that under static conditions, coculture with SMCs induces ICAM-1, VCAM-1, and E-selectin gene expression in ECs. These coculture effects are inhibited by shear stress and require specific interaction between ECs and SMCs in close contact.
- Subjects :
- Vascular smooth muscle
Nitric Oxide Synthase Type III
Endothelium
Myocytes, Smooth Muscle
Immunology
Vascular Cell Adhesion Molecule-1
Cell Communication
Biology
Biochemistry
Umbilical Cord
Cell–cell interaction
Shear stress
medicine
Humans
Myocyte
Regulation of gene expression
Cell adhesion molecule
Cell Biology
Hematology
Intercellular Adhesion Molecule-1
musculoskeletal system
Coculture Techniques
Cell biology
Endothelial stem cell
medicine.anatomical_structure
Gene Expression Regulation
Hemorheology
cardiovascular system
Endothelium, Vascular
Stress, Mechanical
Nitric Oxide Synthase
E-Selectin
Cell Adhesion Molecules
Subjects
Details
- ISSN :
- 15280020 and 00064971
- Volume :
- 101
- Database :
- OpenAIRE
- Journal :
- Blood
- Accession number :
- edsair.doi.dedup.....e39d1200b768fece5ba81c64df4aa3f0
- Full Text :
- https://doi.org/10.1182/blood-2002-08-2560