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Silver nanoparticles promote procoagulant activity of red blood cells: a potential risk of thrombosis in susceptible population
- Source :
- Particle and Fibre Toxicology, Vol 16, Iss 1, Pp 1-14 (2019), Particle and Fibre Toxicology
- Publication Year :
- 2019
- Publisher :
- BMC, 2019.
-
Abstract
- Background Silver nanoparticles (AgNP) are widely used in medical practices owing to their distinct antibacterial, antiviral and anticancer activities. However, with increasing use of AgNP, concerns over its potential toxicity are also escalating. Here, we demonstrated the potential thrombotic effect of AgNP which was mediated by the procoagulant activity of red blood cells (RBCs). Results In freshly isolated human RBCs, AgNP, but not silver microparticles (AgMP), elicited morphological changes, phosphatidylserine (PS) exposure and microvesicles (MV) generation, the key indicators of procoagulant activity in RBCs at concentration ranges (≤ 100 μg/mL) that were free of significant hemolysis. In line with this, AgNP potentiated thrombin generation and adherence of RBCs to endothelial cells, while AgMP did not. Oxidative stress, intracellular calcium increase and ATP depletion were found to underlie the procoagulant effects of AgNP, which led to altered activity of membrane aminophospholipid translocases. These in vitro findings were well reproduced in rat in vivo, where intravenously exposure to AgNP promoted venous thrombosis significantly. Of note, RBCs isolated from cancer patients, who inherently convey the risk of thrombogenesis, were more sensitive to the procoagulant effects of AgNP. In addition, AgNP significantly potentiated the procoagulant effects of a chemotherapeutic drug, paclitaxel. Conclusion Collectively, these results suggest that AgNP may have prothrombotic risks by promoting procoagulant activity of RBCs and caution shall be taken for its use in the population sensitive to thrombosis like cancer patients. Electronic supplementary material The online version of this article (10.1186/s12989-019-0292-6) contains supplementary material, which is available to authorized users.
- Subjects :
- Erythrocytes
Silver
Health, Toxicology and Mutagenesis
Population
lcsh:Industrial hygiene. Industrial welfare
Metal Nanoparticles
Pharmacology
Toxicology
medicine.disease_cause
Silver nanoparticles (AgNP)
chemistry.chemical_compound
In vivo
lcsh:RA1190-1270
Neoplasms
Cell Adhesion
Human Umbilical Vein Endothelial Cells
medicine
Humans
Particle Size
education
Blood Coagulation
Cancer
lcsh:Toxicology. Poisons
education.field_of_study
Toxicity
Research
Red blood cells (RBCs)
Thrombin
Thrombosis
General Medicine
Phosphatidylserine
medicine.disease
Microvesicles
Hemolysis
In vitro
Oxidative Stress
chemistry
Calcium
Oxidative stress
lcsh:HD7260-7780.8
Subjects
Details
- Language :
- English
- ISSN :
- 17438977
- Volume :
- 16
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Particle and Fibre Toxicology
- Accession number :
- edsair.doi.dedup.....e38f77f46d2781107a09f90471b19cbb