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Neutralizing potency and immunochemical evaluation of an anti-Crotalus mictlantecuhtli experimental serum
- Source :
- Toxicon : official journal of the International Society on Toxinology. 187
- Publication Year :
- 2020
-
Abstract
- Snakebite in Mexico is commonly treated with an antivenom which uses Bothrops asper and Crotalus simus venoms as immunogens. Current taxonomic recommendations for the C. simus species complex suggest a novel endemic species from Mexico: Crotalus mictlantecuhtli. The aim of this report was to evaluate the immunogenic properties of C. mictlantecuhtli venom and its potential to generate polyclonal antibodies capable of neutralizing other pitviper venoms. We generated an experimental anti-Crotalus mictlantecuhtli serum, using the rabbit model, to test recognition and neutralizing capacity against the homologous venom as well as venoms from C. atrox, C.basiliscus, C. durissus terrificus, C. scutulatus salvini, C. tzabcan and Ophryacus sphenophrys. Pre-incubation neutralization experiments using our experimental serum showed positive results against venoms containing crotoxin, while venoms from two non-neurotoxic pit-vipers were not neutralized. Rescue experiments in mice showed that, when intravenously injected (i.v.), C. mictlantecuhtli venom is not neutralized by a maximum dose of Antivipmyn® and the experimental serum after 5 min of envenomation, albeit mice envenomated intraperitoneally (i.p.) and rescued i.v. with Antivipmyn® survived even at 50 min after envenomation. Our results highlight the importance of using the highly neurotoxic C. mictlantecuhtli venom to increase antivenom effectiveness against Mexican neurotoxic pitvipers.
- Subjects :
- Immunogen
Antivenom
Bothrops asper
Snake Bites
Venom
Biology
Pharmacology
Toxicology
complex mixtures
Neutralization
03 medical and health sciences
Crotalid Venoms
Animals
Envenomation
Mexico
030304 developmental biology
0303 health sciences
Antivenins
030302 biochemistry & molecular biology
Crotalus
biology.organism_classification
Crotoxin
3. Good health
Crotalus simus
Antibody Formation
Rabbits
Subjects
Details
- ISSN :
- 18793150
- Volume :
- 187
- Database :
- OpenAIRE
- Journal :
- Toxicon : official journal of the International Society on Toxinology
- Accession number :
- edsair.doi.dedup.....e382f836e9aad760beddf9298bcc462d