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Effect of Short-Term Stimulation with Interleukin-1β and Differentiation Medium on Human Mesenchymal Stromal Cell Paracrine Activity in Coculture with Osteoblasts
- Source :
- BioMed Research International, BioMed Research International, Vol 2015 (2015)
- Publication Year :
- 2015
- Publisher :
- Hindawi Publishing Corporation, 2015.
-
Abstract
- Introduction.Human mesenchymal stromal cells (hMSCs) exhibit the potential to accelerate bone healing by enhanced osteogenic differentiation. Interleukin-1βis highly expressed during fracture healing and has been demonstrated to exert a significant impact on the differentiation behaviour of hMSCs. Here, we investigate the effect of 2-hour IL-1βstimulation on the differentiation and paracrine activity of hMSCs in coculture with osteosarcoma cellsin vitro.Methods.hMSCs from 3 donors were incubated for 2 hours with 10 ng/mL IL-1βand subsequently cocultured with MG63-GFP cells either in control or in differentiation medium in a transwell system for 28 days. Genetic and functional effects were investigated.Results.hMSCs cultured in control medium exhibited a regulatory effect on cocultured MG63-GFP cells, resulting in upregulation of osteogenic gene expression in combination with increased ALP activity. However, while stimulated hMSCs cultured under differentiation conditions exhibit signs of osteogenic differentiation, osteogenic differentiation also caused an impaired regulatory effect on the cocultured MG63-GFP cells.Conclusion.Short stimulation of hMSCs has the potential to modify their long-term behaviour. In addition, undifferentiated hMSCs are able to regulate osteoblast differentiation; however, this regulatory function is lost upon osteogenic differentiationin vitro. This offers a novel approach for clinical cell therapy protocols.
- Subjects :
- Adult
Male
endocrine system
Stromal cell
Time Factors
Article Subject
Interleukin-1beta
lcsh:Medicine
Stimulation
600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit
General Biochemistry, Genetics and Molecular Biology
Cell therapy
Downregulation and upregulation
Osteogenesis
Cell Line, Tumor
Paracrine Communication
medicine
Humans
Osteosarcoma
Osteoblasts
General Immunology and Microbiology
Chemistry
lcsh:R
Mesenchymal stem cell
Osteoblast
Cell Differentiation
Mesenchymal Stem Cells
General Medicine
Middle Aged
equipment and supplies
In vitro
Coculture Techniques
Cell biology
Culture Media
medicine.anatomical_structure
Gene Expression Regulation
Cell culture
Immunology
Female
Corrigendum
Research Article
Subjects
Details
- Language :
- English
- ISSN :
- 23146141 and 23146133
- Volume :
- 2015
- Database :
- OpenAIRE
- Journal :
- BioMed Research International
- Accession number :
- edsair.doi.dedup.....e3614b359abf0763fd4ea73218a220c2