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Identification of differentially expressed genes and their subpathways in recurrent versus primary bone giant cell tumors
- Source :
- International journal of oncology. 45(3)
- Publication Year :
- 2014
-
Abstract
- Giant cell tumor (GCT) of the bone is a benign but locally aggressive bone neoplasm with a strong tendency to develop local recurrent and metastatic disease. Thus, it provides a useful model system for the identification of biological mechanisms involved in bone tumor progression and metastasis. This study profiled 24 cases of recurrent versus primary bone GCT tissues using QuantiGene 2.0 Multiplex Arrays that included Human p53 80-Plex Panels and Human Stem Cell 80-Plex Panels. A total of 32 differentially expressed genes were identified, including the 20 most upregulated genes and the 12 most downregulated genes in recurrent GCT. The genes identified are related to cell growth, adhesion, apoptosis, signal transduction and bone formation. Furthermore, iSubpathwayMiner analyses were performed to identify significant biological pathway regions (subpathway) associated with this disease. The pathway analysis identified 11 statistically significant enriched subpathways, including pathways in cancer, p53 signaling pathway, osteoclast differentiation pathway and Wnt signaling pathway. Among these subpathways, four genes (IGF1, MDM2, STAT1 and RAC1) were presumed to play an important role in bone GCT recurrence. The differentially expressed MDM2 protein was immunohistochemically confirmed in the recurrent versus primary bone GCT tissues. This study identified differentially expressed genes and their subpathways in recurrent GCT, which may serve as potential biomarkers for the prediction of GCT recurrence.
- Subjects :
- Bone neoplasm
Adult
Male
Cancer Research
Pathology
medicine.medical_specialty
Adolescent
Bone Neoplasms
Biology
Metastasis
Young Adult
Osteoclast
medicine
Humans
Giant Cell Tumors
Oligonucleotide Array Sequence Analysis
Giant Cell Tumor of Bone
Gene Expression Profiling
Wnt signaling pathway
Middle Aged
medicine.disease
Gene Expression Regulation, Neoplastic
medicine.anatomical_structure
Primary bone
Oncology
Tumor progression
Neoplasm Recurrence, Local
Giant-cell tumor of bone
Signal Transduction
Subjects
Details
- ISSN :
- 17912423
- Volume :
- 45
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- International journal of oncology
- Accession number :
- edsair.doi.dedup.....e360623205b88da2aedcdc923bccd2ea