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Runx2 expression is associated with pathologic new bone formation around radicular cysts: an immunohistochemical demonstration
- Source :
- Journal of Oral Pathology and Medicine. 35:492-499
- Publication Year :
- 2006
- Publisher :
- Wiley, 2006.
-
Abstract
- Background: Radicular cysts are the most common cysts in human jaw bones. These lesions induce bone remodeling of the surrounding alveolar bones, which was termed ‘condensing osteitis’, and was suggested to be related to cells of the osteoblastic lineage. The Runx2 (core-binding protein [cbfa]1/polyoma enhancer-binding protein [pebp]2αA) was shown to be a DNA-binding transcriptional molecule expressed in osteoprogenitor cells. Methods: We confirmed the specificity of anti-Runx2 antiserum, using Western blotting analysis. We investigated the expression and localization of Runx2 in 32 radicular cyst cases with bone tissue fragments, immunohistochemically. Results: Signals for Runx2 were seen in 18 cases (56.3%) of radicular cysts with bone formation. These signals were immunolocalized in the nuclei of the spindle-shaped osteoprogenitor cells in the cyst walls, whereas only a few signals were seen in the cuboidal osteoblastic cells near the fibrous bones. Signals for type I collagen were immunolocalized in the dense collagen fibers in the cyst walls and in the matrix of the fibrous bone around the radicular cysts, whereas no signals were seen on the inner portions with inflammatory cell infiltration of the cyst walls. Very weak signals for transforming growth factor (TGF)-β1 were infrequently seen in the osteoblasts of the fibrous bone, whereas signals for TGF-β2 were observed in young osteocytes in the fibrous bones, in B-cell lymphocytes infiltrating into the inner portions, and on the cellular membranes of the lining epithelium. Conclusions: The nuclear expression of Runx2 in spindle-shaped cells in the outer portions may play an essential role in the induction of fibrous bone tissue around radicular cysts. TGF-β2 may play a role in the production of type I collagen, which acts as a template for pathologic new bone formation, in radicular cysts.
- Subjects :
- Male
musculoskeletal diseases
Cancer Research
Pathology
medicine.medical_specialty
Receptor, Transforming Growth Factor-beta Type I
Condensing osteitis
Core Binding Factor Alpha 1 Subunit
Protein Serine-Threonine Kinases
Biology
Matrix (biology)
Bone tissue
Collagen Type I
Pathology and Forensic Medicine
Bone remodeling
Mice
Bone cell
medicine
Animals
Humans
Cyst
Radicular Cyst
Ossification, Heterotopic
Receptor, Transforming Growth Factor-beta Type II
Anatomy
Middle Aged
Antigens, CD20
medicine.disease
Platelet Endothelial Cell Adhesion Molecule-1
medicine.anatomical_structure
Otorhinolaryngology
Leukocyte Common Antigens
Periodontics
Female
Bone Remodeling
Oral Surgery
Activin Receptors, Type I
Receptors, Transforming Growth Factor beta
Type I collagen
Subjects
Details
- ISSN :
- 16000714 and 09042512
- Volume :
- 35
- Database :
- OpenAIRE
- Journal :
- Journal of Oral Pathology and Medicine
- Accession number :
- edsair.doi.dedup.....e33b7dfad81e4e6edd81512a0c9bbd19