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The transcription factors Sox5 and Sox9 regulate Catsper1 gene expression
- Source :
- FEBS Letters. (18):3352-3360
- Publisher :
- Federation of European Biochemical Societies. Published by Elsevier B.V.
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Abstract
- Catsper is a Ca2+permeable channel required for sperm hyperactivation. In spite of its central role in male fertility, the transcriptional mechanisms that regulate Catsper1 expression are ill defined. In this work, we describe the identification and characterization of important regulatory elements in the murine Catsper1 gene proximal promoter. Four transcription start sites and three functional Sox-binding sites were identified in the Catsper1 promoter. Interestingly, transcription factors Sox5 and Sox9 caused a significant increase in transactivation of the Catsper1 promoter in heterologous systems, and chromatin immunoprecipitation assays showed that both transcription factors interact with the Catsper1 promoter in vivo. These results provide new insights into the molecular mechanisms that control Catsper channel expression.
- Subjects :
- Male
Transcription, Genetic
Molecular Sequence Data
Response element
Biophysics
Biology
Biochemistry
Mice
Transactivation
Transcriptional regulation
Spermatocytes
Structural Biology
Testis
Genetics
Animals
Humans
Promoter Regions, Genetic
Molecular Biology
Transcription factor
Binding Sites
Base Sequence
General transcription factor
Hyperactivation
SOX9 Transcription Factor
Promoter
Cell Biology
Spermatids
Catsper1
Calcium channels
HEK293 Cells
Gene Expression Regulation
Transcription Initiation Site
Sox5
SOXD Transcription Factors
Chromatin immunoprecipitation
Protein Binding
Sox9
Subjects
Details
- Language :
- English
- ISSN :
- 00145793
- Issue :
- 18
- Database :
- OpenAIRE
- Journal :
- FEBS Letters
- Accession number :
- edsair.doi.dedup.....e33aac07ac118f7873cdae606bcd2ed1
- Full Text :
- https://doi.org/10.1016/j.febslet.2014.07.024