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Deregulated Local Protein Synthesis in the Brain Synaptosomes of a Mouse Model for Alzheimer’s Disease
- Source :
- Molecular Neurobiology. 57:1529-1541
- Publication Year :
- 2019
- Publisher :
- Springer Science and Business Media LLC, 2019.
-
Abstract
- While protein synthesis in neurons is largely attributed to cell body and dendrites, the capability of synaptic regions to synthesize new proteins independently of the cell body has been widely demonstrated as an advantageous mechanism subserving synaptic plasticity. Thus, the contribution that local protein synthesis at synapses makes to physiology and pathology of brain plasticity may be more prevalent than initially thought. In this study, we tested if local protein synthesis at synapses is deregulated in the brains of TgCRND8 mice, an animal model for Alzheimer’s disease (AD) overexpressing mutant human amyloid precursor protein (APP). To this end, we used synaptosomes as a model system to study the functionality of the synaptic regions in mouse brains. Our results showed that, while TgCRND8 mice exhibit early signs of brain inflammation and deficits in learning, the electrophoretic profile of newly synthesized proteins in their synaptosomes was subtly different from that of the control mice. Interestingly, APP itself was, in part, locally synthesized in the synaptosomes, underscoring the potential importance of local translation at synapses. More importantly, after the contextual fear conditioning, de novo synthesis of some individual proteins was significantly enhanced in the synaptosomes of control animals, but the TgCRND8 mice failed to display such synaptic modulation by training. Taken together, our results demonstrate that synaptic synthesis of proteins is impaired in the brain of a mouse model for AD, and raise the possibility that this deregulation may contribute to the early progression of the pathology.
- Subjects :
- 0301 basic medicine
Mutant
Cell
Neuroscience (miscellaneous)
Mice, Transgenic
Plaque, Amyloid
Inflammation
Biology
Synaptic plasticity
Amyloid beta-Protein Precursor
03 medical and health sciences
Cellular and Molecular Neuroscience
0302 clinical medicine
Alzheimer Disease
Neuroplasticity
Amyloid precursor protein
medicine
Protein biosynthesis
Learning
Animals
Neurons
Memory Disorders
Amyloid beta-Peptides
Local protein synthesi
Brain
Translation (biology)
Disease Models, Animal
030104 developmental biology
medicine.anatomical_structure
Neurology
Synapses
biology.protein
medicine.symptom
Alzheimer’s disease
Neuroscience
030217 neurology & neurosurgery
Synaptosomes
Subjects
Details
- ISSN :
- 15591182 and 08937648
- Volume :
- 57
- Database :
- OpenAIRE
- Journal :
- Molecular Neurobiology
- Accession number :
- edsair.doi.dedup.....e31c71953f00226aa6524855ca864e78