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Design of homo-organic acid producing strains using multi-objective optimization
- Source :
- Metabolic Engineering. 28:63-73
- Publication Year :
- 2015
- Publisher :
- Elsevier BV, 2015.
-
Abstract
- Production of homo-organic acids without byproducts is an important challenge in bioprocess engineering to minimize operation cost for separation processes. In this study, we used multi-objective optimization to design Escherichia coli strains with the goals of maximally producing target organic acids, while maintaining sufficiently high growth rate and minimizing the secretion of undesired byproducts. Homo-productions of acetic, lactic and succinic acids were targeted as examples. Engineered E. coli strains capable of producing homo-acetic and homo-lactic acids could be developed by taking this systems approach for the minimal identification of gene knockout targets. Also, failure to predict effective gene knockout targets for the homo-succinic acid production suggests that the multi-objective optimization is useful in assessing the suitability of a microorganism as a host strain for the production of a homo-organic acid. The systems metabolic engineering-based approach reported here should be applicable to the production of other industrially important organic acids.
- Subjects :
- chemistry.chemical_classification
Carboxylic Acids
Bioengineering
Biology
medicine.disease_cause
Applied Microbiology and Biotechnology
Multi-objective optimization
Metabolic engineering
chemistry.chemical_compound
Metabolic Engineering
chemistry
Biochemistry
Bioprocess engineering
Succinic acid
Gene Knockdown Techniques
Escherichia coli
medicine
Fermentation
Gene knockout
Biotechnology
Organic acid
Subjects
Details
- ISSN :
- 10967176
- Volume :
- 28
- Database :
- OpenAIRE
- Journal :
- Metabolic Engineering
- Accession number :
- edsair.doi.dedup.....e30effff3ab7c61e46c237d190d33317