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Cucumber mosaic virus as the expression system for a potential vaccine against Alzheimer's disease
- Source :
- Journal of virological methods 169 (2010): 332–340. doi:10.1016/j.jviromet.2010.07.039, info:cnr-pdr/source/autori:Vitti A.1, Piazzolla G. 2, Condelli V.1, Nuzzaci M.1,Lanorte M.T. 1, Boscia D., De Stradis A., Antonaci S.2, Piazzolla P.1, Tortorella C.2/titolo:Cucumber mosaic virus as the expression system for a potential vaccine against Alzheimer's disease/doi:10.1016%2Fj.jviromet.2010.07.039/rivista:Journal of virological methods/anno:2010/pagina_da:332/pagina_a:340/intervallo_pagine:332–340/volume:169
- Publication Year :
- 2010
- Publisher :
- Elsevier Science Publishers :, Amsterdam , Paesi Bassi, 2010.
-
Abstract
- A primary therapeutic goal in Alzheimers disease (AD) is to reduce the quantity of amyloid b protein (A b) present in the brain. To develop an effective, safe system for vaccination against Alzheimers disease, the plant virus Cucumber mosaic virus (CMV) was engineered genetically to express A b -derived fragments that stimulate mainly humoral immune responses. Six chimeric constructs, bearing the A b 115 or the A b 415 sequence in positions 248, 392 or 529 of the CMV coat protein (CP) gene, were created. Viral products proved to be able to replicate in their natural host. However, only chimeric A b 115-CMVs were detected by A b 142 antiserum in Western blot analysis. Experimental evidence of Immunoelectron microscopy revealed a complete decoration of A b 115-CMV248 and A b 15-CMV392 following incubation with either anti-A b 115 or anti-A b 142 polyclonal antibodies. These two chimeric CMVs appear to be endowed with features making them possible candidates for vaccination against Alzheimers disease.
- Subjects :
- Models, Molecular
Virus Cultivation
Alzheimer Vaccines
Immunoelectron microscopy
Genetic Vectors
Molecular Sequence Data
Gene Expression
Biology
A ² 1-15
Cucumovirus
Virus
Cucumber mosaic virus
Immune system
Alzheimer Disease
Virology
Plant virus
Tobacco
Humans
Amino Acid Sequence
Microscopy, Immunoelectron
Antiserum
Amyloid beta-Peptides
Chimeric plant viruses
Amyloid ²
Alzheimer's disease
biology.organism_classification
Protein Structure, Tertiary
Humoral immunity
Capsid Proteins
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Journal of virological methods 169 (2010): 332–340. doi:10.1016/j.jviromet.2010.07.039, info:cnr-pdr/source/autori:Vitti A.1, Piazzolla G. 2, Condelli V.1, Nuzzaci M.1,Lanorte M.T. 1, Boscia D., De Stradis A., Antonaci S.2, Piazzolla P.1, Tortorella C.2/titolo:Cucumber mosaic virus as the expression system for a potential vaccine against Alzheimer's disease/doi:10.1016%2Fj.jviromet.2010.07.039/rivista:Journal of virological methods/anno:2010/pagina_da:332/pagina_a:340/intervallo_pagine:332–340/volume:169
- Accession number :
- edsair.doi.dedup.....e2fdc817ffe264cda588678bf8651fdc