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Development of a nanotechnology-based approach for capturing and detecting nucleic acids by using flow cytometry
- Source :
- Talanta
- Publication Year :
- 2021
-
Abstract
- Nucleic acid-based molecular diagnosis has gained special importance for the detection and early diagnosis of genetic diseases as well as for the control of infectious disease outbreaks. The development of systems that allow for the detection and analysis of nucleic acids in a low-cost and easy-to-use way is of great importance. In this context, we present a combination of a nanotechnology-based approach with the already validated dynamic chemical labeling (DCL) technology, capable of reading nucleic acids with single-base resolution. This system allows for the detection of biotinylated molecular products followed by simple detection using a standard flow cytometer, a widely used platform in clinical and molecular laboratories, and therefore, is easy to implement. This proof-of-concept assay has been developed to detect mutations in KRAS codon 12, as these mutations are highly important in cancer development and cancer treatments.<br />Graphical abstract Image 1<br />Highlights • A nanotechnology-based approach for capturing and detecting nucleic acids by flow cytometry has been developed. • A great advantage is that molecular reactions are taking place in solution while the reading is done by using flow cytometry. • Detection of KRAS codon 12 and miR-122 sequences using this approach has been successfully carried out. • This approach will have a clear impact in molecular diagnostic.
- Subjects :
- Peptide Nucleic Acids
Nucleic acid testing (NAT)
Fluorescence-activated cell sorting (FACS)
Nanotechnology
Context (language use)
02 engineering and technology
01 natural sciences
Article
Analytical Chemistry
Flow cytometry
Dynamic chemical labeling (DCL)
Nucleic Acids
medicine
medicine.diagnostic_test
Chemistry
010401 analytical chemistry
Peptide nucleic acid (PNA)
021001 nanoscience & nanotechnology
Flow Cytometry
0104 chemical sciences
Infectious disease (medical specialty)
Biotinylation
Mutation
Nucleic acid
KRAS point Mutation
Cancer development
0210 nano-technology
Chemical labeling
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Talanta
- Accession number :
- edsair.doi.dedup.....e2e998a99c85b319b35903ad344be327