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Surface Display of Human Growth Hormone on Bacillus subtilis Spores for Oral Administration
- Source :
- Current Microbiology. 68:463-471
- Publication Year :
- 2013
- Publisher :
- Springer Science and Business Media LLC, 2013.
-
Abstract
- Human growth hormone (hGH) is the major and important hormone component of human being. At present, hGH for clinical uses is mostly produced in Escherichia coli, which requires costly denaturation and refolding to recover functionality. To obtain long-term bioactive hormone, we used hGH as a foreign gene and constructed a recombinant plasmid pJS700-hGH which carries a recombinant gene cotC-hgh with an enterokinase site under the control of cotC promoter. Plasmid pJS700-hGH was transformed into Bacillus subtilis by double crossover and an amylase-inactivated mutant was produced. After spore formation, Western blot and fluorescence immunoassay were used to monitor hGH surface expression on spores. Oral administration to silkworm with spores displaying hGH further showed that the recombinant spores may have potential ability to be digested and absorbed into the silkworm's hemolymph due to both the resistant characters of spores and the addition of enterokinase site.
- Subjects :
- Spores
Enteropeptidase
endocrine system
Recombinant Fusion Proteins
Mutant
Administration, Oral
Bacillus subtilis
Protein Engineering
Transfection
medicine.disease_cause
Applied Microbiology and Biotechnology
Microbiology
law.invention
Plasmid
law
Sporogenesis
medicine
Animals
Humans
Cloning, Molecular
Escherichia coli
biology
Human Growth Hormone
fungi
General Medicine
Bombyx
biology.organism_classification
Molecular biology
Spore
embryonic structures
Recombinant DNA
Cell Surface Display Techniques
hormones, hormone substitutes, and hormone antagonists
Subjects
Details
- ISSN :
- 14320991 and 03438651
- Volume :
- 68
- Database :
- OpenAIRE
- Journal :
- Current Microbiology
- Accession number :
- edsair.doi.dedup.....e2c9d53fdd299d9013f1be4cf44a0624