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Developmentally regulated expression of calcitonin gene-related peptide at mammalian neuromuscular junction
- Source :
- Journal of molecular neuroscience : MN. 2(3)
- Publication Year :
- 1990
-
Abstract
- Using indirect immunofluorescence techniques, we have found that calcitonin gene-related peptide-like immunoreactivity is present in the neuromuscular junctions of somatic muscles as well as in almost all motor neurons of the lumbar enlargement of 1-week-old rats. It gradually decreases in both motor neurons and motor nerve endings as the animal grows up and completely disappears from the neuromuscular junctions in adult rats, persisting only in the motor nerve endings on the intrafusal fibers. In situ hybridization experiments have shown that the down-regulation of calcitonin gene-related peptide-like immunoreactivity is strictly related to a reduction in CGRP mRNA levels in the spinal motor neurons. These results indicate that the expression of CGRP is developmentally regulated in spinal cord alpha motor neurons. They also suggest that the peptide may play an important role at the immature neuromuscular junction.
- Subjects :
- medicine.medical_specialty
Aging
Aging, Animals, Calcitonin Gene-Related Peptide
analysis/genetics, Fluorescent Antibody Technique, Gene Expression Regulation, Motor Neurons
metabolism, Muscle Development, Muscles
metabolism, Neuromuscular Junction
metabolism, Rats, Rats
Inbred Strains, Receptors
Cholinergic
analysis, Spinal Cord
growth /&/ development/metabolism
analysis
Calcitonin Gene-Related Peptide
Inbred Strains
Neuromuscular Junction
Motor nerve
Fluorescent Antibody Technique
analysis/genetics
In situ hybridization
Calcitonin gene-related peptide
Biology
Muscle Development
Neuromuscular junction
Lumbar enlargement
Cellular and Molecular Neuroscience
Internal medicine
Receptors
medicine
Animals
Receptors, Cholinergic
Motor Neurons
Muscles
Rats, Inbred Strains
General Medicine
Spinal cord
Rats
Endocrinology
medicine.anatomical_structure
Gene Expression Regulation
Spinal Cord
Calcitonin
metabolism
Subjects
Details
- ISSN :
- 08958696
- Volume :
- 2
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Journal of molecular neuroscience : MN
- Accession number :
- edsair.doi.dedup.....e2b8264df201029465519197b12a9a9a