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Functionalized Mesoporous Silica Nanoparticle with Antioxidants as a New Carrier That Generates Lower Oxidative Stress Impact on Cells
- Source :
- Molecular Pharmaceutics, Molecular Pharmaceutics, American Chemical Society, 2016, 13 (8), pp.2647-2660. ⟨10.1021/acs.molpharmaceut.6b00190⟩, Molecular Pharmaceutics, 2016, 13 (8), pp.2647-2660. ⟨10.1021/acs.molpharmaceut.6b00190⟩
- Publication Year :
- 2016
- Publisher :
- HAL CCSD, 2016.
-
Abstract
- UMR AGAP équipe DAAV Diversité, adaptation et amélioration de la vigne; International audience; Mesoporous silica nanoparticles (MSNs) were covalently coated with antioxidant molecules, namely, caffeic acid (MSN-CAF) or rutin (MSN-RUT), in order to diminish the impact of oxidative stress induced after transfection into cells, thus generating safer carriers used for either drug delivery or other applications. Two cellular models involved in the entry of NPs in the body were used for this purpose: the intestinal Caco-2 and the epidermal HaCaT cell lines. Rutin gave the best results in terms of antioxidant capacities preservation during coupling procedures, cellular toxicity alleviation, and decrease of ROS level after 24 h incubation of cells with grafted nanoparticles. These protective effects of rutin were found more pronounced in HaCaT than in Caco-2 cells, indicating some cellular specificity toward defense against oxidative stress. In order to gain more insight about the Nrf2 response, a stable transfected HaCaT cell line bearing repeats of the antioxidant response element (ARE) in front of a luciferase reporter gene was generated. In this cell line, both tBHQ and quercetin (Nrf2 agonists), but not rutin, were able to induce, in a dose-dependent fashion, the luciferase response. Interestingly, at high concentration, MSN-RUT was able to induce a strong Nrf2 protective response in HaCaT cells, accompanied by a comparable induction of HO-1 mRNA. The level of these responses was again less important in Caco-2 cells. To conclude, in keratinocyte cell line, the coupling of rutin to silica nanoparticles was beneficial in term of ROS reduction, cellular viability, and protective effects mediated through the activation of the Nrf2 antioxidant pathway.
- Subjects :
- 0301 basic medicine
Antioxidant
Cell Survival
NF-E2-Related Factor 2
medicine.medical_treatment
[SDV]Life Sciences [q-bio]
Catechols
Pharmaceutical Science
02 engineering and technology
medicine.disease_cause
Polymerase Chain Reaction
Antioxidants
Nrf2
catechol antioxidant
03 medical and health sciences
Rutin
chemistry.chemical_compound
Caco-2 cell line
Cell Line, Tumor
Drug Discovery
medicine
Humans
antioxidative response
Chemistry
Transfection
021001 nanoscience & nanotechnology
Silicon Dioxide
Hydroquinones
[SDV] Life Sciences [q-bio]
HaCaT
Oxidative Stress
030104 developmental biology
medicine.anatomical_structure
Biochemistry
Cell culture
HaCaT cell line
Biophysics
mesoporous silica nanoparticle
Molecular Medicine
Nanoparticles
Quercetin
Caco-2 Cells
0210 nano-technology
Keratinocyte
Oxidative stress
Subjects
Details
- Language :
- English
- ISSN :
- 15438384 and 15438392
- Database :
- OpenAIRE
- Journal :
- Molecular Pharmaceutics, Molecular Pharmaceutics, American Chemical Society, 2016, 13 (8), pp.2647-2660. ⟨10.1021/acs.molpharmaceut.6b00190⟩, Molecular Pharmaceutics, 2016, 13 (8), pp.2647-2660. ⟨10.1021/acs.molpharmaceut.6b00190⟩
- Accession number :
- edsair.doi.dedup.....e2aa0d081089a5bc76ce4f0239bb442c