Back to Search
Start Over
iASPP, a microRNA‑124 target, is aberrantly expressed in astrocytoma and regulates�malignant�glioma�cell�migration and viability
- Source :
- Molecular Medicine Reports.
- Publication Year :
- 2017
- Publisher :
- Spandidos Publications, 2017.
-
Abstract
- MicroRNAs (miRNAs) regulate biogenesis and disease development by targeting numerous mRNAs. miRNA (miR)‑124 and its direct target, inhibitor of apoptosis‑stimulating protein of p53 (iASPP), may be involved in tumor development and progression. The aim of the present study was to explore the role of miR‑124‑targeted iASPP in glioma. The results demonstrated that miR‑124 was aberrantly expressed in astrocytic glioma tissue and in the human glioblastoma cell lines U87 and U251. The expression of miR‑124 was lower in astrocytic gliomas compared with normal brain (NB) tissues, with a more reduced expression in higher‑grade tumors. In addition, several miR‑124 loci (including miR‑124‑1, miR‑124‑2 and miR‑124‑3) were revealed to be more highly methylated in U87 cells compared with methylation levels in U251 cells and NB cells. Furthermore, the expression of iASPP was higher in high‑grade astrocytic gliomas compared with low‑grade astrocytic gliomas. miR‑124 overexpression effectively inhibited U87 and U251 cell migration. In addition, miR‑124 regulated cell viability and arrested the cell cycle at the G0/G1 phase in these two cell lines. miR‑124 also reduced the expression levels of the cell cycle related genes iASPP, cyclin‑dependent kinase (CDK)4, CDK6 and cyclin D1. Results from the present study indicated that expression of the miR‑124 target gene iASPP may contribute to glioma development and progression.
- Subjects :
- Male
0301 basic medicine
Cancer Research
Cell Survival
Astrocytoma
Biochemistry
03 medical and health sciences
0302 clinical medicine
Cyclin D1
Cell Movement
Cyclin-dependent kinase
Cell Line, Tumor
Glioma
microRNA
Genetics
medicine
Humans
Molecular Biology
Cell Proliferation
biology
Oncogene
Brain Neoplasms
Cell growth
Intracellular Signaling Peptides and Proteins
Middle Aged
Cell cycle
medicine.disease
Gene Expression Regulation, Neoplastic
Repressor Proteins
MicroRNAs
030104 developmental biology
Oncology
030220 oncology & carcinogenesis
biology.protein
Cancer research
Molecular Medicine
Female
Cyclin-dependent kinase 6
Glioblastoma
Subjects
Details
- ISSN :
- 17913004 and 17912997
- Database :
- OpenAIRE
- Journal :
- Molecular Medicine Reports
- Accession number :
- edsair.doi.dedup.....e29618f467bc5c19ba366b52026b9e38
- Full Text :
- https://doi.org/10.3892/mmr.2017.8097