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Effect of Chum Salmon Egg Lectin on Tight Junctions in Caco-2 Cell Monolayers
- Source :
- Molecules, Volume 20, Issue 5, Pages 8094-8106, Molecules, Vol 20, Iss 5, Pp 8094-8106 (2015)
- Publication Year :
- 2015
- Publisher :
- Multidisciplinary Digital Publishing Institute, 2015.
-
Abstract
- The effect of a chum salmon egg lectin (CSL3) on tight junction (TJ) of Caco-2 cell monolayers was investigated. The lectin opened TJ as indicated by the decrease of the transepithelial electrical resistance (TER) value and the increase of the permeation of lucifer yellow, which is transported via the TJ-mediated paracellular pathway. The effects of CSL3 were inhibited by the addition of 10 mM L-rhamnose or D-galactose which were specific sugars for CSL3. The lectin increased the intracellular Ca2+ of Caco-2 cell monolayers, that could be inhibited by the addition of L-rhamnose. The fluorescence immunostaining of β-actin in Caco-2 cell monolayers revealed that the cytoskeleton was changed by the CSL3 treatment, suggesting that CSL3 depolymerized β-actin to cause reversible TJ structural and functional disruption. Although Japanese jack bean lectin and wheat germ lectin showed similar effects in the decrease of the TER values and the increase of the intracellular Ca2+, they could not be inhibited by the same concentrations of simple sugars, such as D-glucose and N-acetyl-D-glucosamine.
- Subjects :
- Fish Proteins
tight junction
Pharmaceutical Science
Rhamnose
Article
Analytical Chemistry
Tight Junctions
lcsh:QD241-441
Caco-2 cell
chemistry.chemical_compound
lcsh:Organic chemistry
Cell Line, Tumor
Lectins
Drug Discovery
Humans
Physical and Theoretical Chemistry
Cytoskeleton
intestinal transport
transepithelial transport
Lucifer yellow
biology
Tight junction
Organic Chemistry
Egg Proteins
Lectin
Galactose
Membrane Proteins
Biological Transport
Actins
Glucose
chemistry
Biochemistry
Chemistry (miscellaneous)
Caco-2
Cell culture
Paracellular transport
biology.protein
Biophysics
Molecular Medicine
lectin
Calcium
Caco-2 Cells
Intracellular
Subjects
Details
- Language :
- English
- ISSN :
- 14203049
- Database :
- OpenAIRE
- Journal :
- Molecules
- Accession number :
- edsair.doi.dedup.....e294564d952528a26be1996715d0d61c
- Full Text :
- https://doi.org/10.3390/molecules20058094