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Effect of Chum Salmon Egg Lectin on Tight Junctions in Caco-2 Cell Monolayers

Authors :
Shintaro Yamamoto
Koji Muramoto
Ryno J. Naudé
Tomohisa Ogawa
Ryo Nemoto
Source :
Molecules, Volume 20, Issue 5, Pages 8094-8106, Molecules, Vol 20, Iss 5, Pp 8094-8106 (2015)
Publication Year :
2015
Publisher :
Multidisciplinary Digital Publishing Institute, 2015.

Abstract

The effect of a chum salmon egg lectin (CSL3) on tight junction (TJ) of Caco-2 cell monolayers was investigated. The lectin opened TJ as indicated by the decrease of the transepithelial electrical resistance (TER) value and the increase of the permeation of lucifer yellow, which is transported via the TJ-mediated paracellular pathway. The effects of CSL3 were inhibited by the addition of 10 mM L-rhamnose or D-galactose which were specific sugars for CSL3. The lectin increased the intracellular Ca2+ of Caco-2 cell monolayers, that could be inhibited by the addition of L-rhamnose. The fluorescence immunostaining of β-actin in Caco-2 cell monolayers revealed that the cytoskeleton was changed by the CSL3 treatment, suggesting that CSL3 depolymerized β-actin to cause reversible TJ structural and functional disruption. Although Japanese jack bean lectin and wheat germ lectin showed similar effects in the decrease of the TER values and the increase of the intracellular Ca2+, they could not be inhibited by the same concentrations of simple sugars, such as D-glucose and N-acetyl-D-glucosamine.

Details

Language :
English
ISSN :
14203049
Database :
OpenAIRE
Journal :
Molecules
Accession number :
edsair.doi.dedup.....e294564d952528a26be1996715d0d61c
Full Text :
https://doi.org/10.3390/molecules20058094