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On-line comprehensive two-dimensional normal-phase liquid chromatography×reversed-phase liquid chromatography for preparative isolation of toad venom
- Source :
- Journal of Chromatography A. 1456:169-175
- Publication Year :
- 2016
- Publisher :
- Elsevier BV, 2016.
-
Abstract
- An on-line comprehensive preparative two-dimensional normal-phase liquid chromatography×reversed-phase liquid chromatography (2D NPLC×RPLC) system was constructed with a newly developed vacuum evaporation assisted adsorption (VEAA) interface, allowing fast removal of NPLC solvent in the vacuum condition and successfully solving the solvent incompatibility problem between NPLC and RPLC. The system achieved on-line solvent exchange within the two dimensions and its performance was illustrated by gram-scale isolation of crude extract from the venom of Bufo bufo gargarizans. Within separation time of ∼20h, 19 compounds were obtained with high purity in a single run. With the VEAA interface, the 2D system exhibited apparent advantages in separation efficiency and automation compared with conventional methods, indicating its promising application in the routine separation process for complicated natural products.
- Subjects :
- Spectrometry, Mass, Electrospray Ionization
Magnetic Resonance Spectroscopy
Chemical substance
Analytical chemistry
01 natural sciences
Biochemistry
Bufo bufo
Analytical Chemistry
Vacuum evaporation
Toad Venom
Automation
Adsorption
Animals
Chromatography, High Pressure Liquid
Chromatography, Reverse-Phase
Chromatography
010405 organic chemistry
Chemistry
010401 analytical chemistry
Organic Chemistry
Temperature
General Medicine
Reversed-phase chromatography
0104 chemical sciences
Separation process
Bufanolides
Solvent
Amphibian Venoms
Chromatography, Liquid
Bufo bufo gargarizans
Subjects
Details
- ISSN :
- 00219673
- Volume :
- 1456
- Database :
- OpenAIRE
- Journal :
- Journal of Chromatography A
- Accession number :
- edsair.doi.dedup.....e28ea710444284903209b0ba9c01af2e