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Adipose-specific Lipoprotein Lipase Deficiency More Profoundly Affects Brown than White Fat Biology
- Source :
- The Journal of Biological Chemistry, 288(20), 14046-14058. AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC, Journal of biological chemistry, 288(20), 14046-14058. American Society for Biochemistry and Molecular Biology Inc.
- Publication Year :
- 2013
- Publisher :
- Elsevier BV, 2013.
-
Abstract
- Adipose fat storage is thought to require uptake of circulating triglyceride (TG)-derived fatty acids via lipoprotein lipase (LpL). To determine how LpL affects the biology of adipose tissue, we created adipose-specific LpL knock-out (ATLO) mice, and we compared them with whole body LpL knock-out mice rescued with muscle LpL expression (MCK/L0) and wild type (WT) mice. ATLO LpL mRNA and activity were reduced, respectively, 75 and 70% in gonadal adipose tissue (GAT), 90 and 80% in subcutaneous tissue, and 84 and 85% in brown adipose tissue (BAT). ATLO mice had increased plasma TG levels associated with reduced chylomicron TG uptake into BAT and lung. ATLO BAT, but not GAT, had altered TG composition. GAT from MCK/L0 was smaller and contained less polyunsaturated fatty acids in TG, although GAT from ATLO was normal unless LpL was overexpressed in muscle. High fat diet feeding led to less adipose in MCK/L0 mice but TG acyl composition in subcutaneous tissue and BAT reverted to that of WT. Therefore, adipocyte LpL in BAT modulates plasma lipoprotein clearance, and the greater metabolic activity of this depot makes its lipid composition more dependent on LpL-mediated uptake. Loss of adipose LpL reduces fat accumulation only if accompanied by greater LpL activity in muscle. These data support the role of LpL as the "gatekeeper" for tissue lipid distribution.
- Subjects :
- Adipose Tissue/metabolism
Male
Adipose Tissue, Brown/metabolism
Adipose tissue
White adipose tissue
Biochemistry
Mice
chemistry.chemical_compound
Adipose Tissue, Brown
Adipocyte
Chylomicrons
Brown adipose tissue
Adipocytes
Bone Marrow Transplantation
Mice, Knockout
Lipoprotein lipase
Adipocytes/cytology
digestive, oral, and skin physiology
Lipids
White/metabolism
Triglycerides/blood
Phenotype
medicine.anatomical_structure
Adipose Tissue
lipids (amino acids, peptides, and proteins)
medicine.medical_specialty
Chylomicrons/pharmacokinetics
Adipose Tissue, White/metabolism
Knockout
Adipose Tissue, White
Lipolysis
Lipids/chemistry
Biology
Brown/metabolism
Internal medicine
medicine
Animals
Molecular Biology
Triglycerides
Triglyceride
Macrophages
Macrophages/cytology
nutritional and metabolic diseases
Cell Biology
Lipoprotein Lipase/deficiency
Lipoprotein Lipase
Endocrinology
chemistry
Chylomicron
Subjects
Details
- ISSN :
- 00219258
- Volume :
- 288
- Database :
- OpenAIRE
- Journal :
- Journal of Biological Chemistry
- Accession number :
- edsair.doi.dedup.....e28a31188ddf42ff821892409a23ea00