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Prelamin A is involved in early steps of muscle differentiation
- Source :
- Experimental Cell Research. 314:3628-3637
- Publication Year :
- 2008
- Publisher :
- Elsevier BV, 2008.
-
Abstract
- Lamin A is a nuclear lamina constituent implicated in a number of human disorders including Emery-Dreifuss muscular dystrophy. Since increasing evidence suggests a role of the lamin A precursor in nuclear functions, we investigated the processing of prelamin A during differentiation of C2C12 mouse myoblasts. We show that both protein levels and cellular localization of prelamin A are modulated during myoblast activation. Similar changes of lamin A-binding proteins emerin and LAP2alpha were observed. Furthermore, prelamin A was found in a complex with LAP2alpha in differentiating myoblasts. Prelamin A accumulation in cycling myoblasts by expressing unprocessable mutants affected LAP2alpha and PCNA amount and increased caveolin 3 mRNA and protein levels, while accumulation of prelamin A in differentiated muscle cells following treatment with a farnesyl transferase inhibitor appeared to inhibit caveolin 3 expression. Our data provide evidence for a critical role of the lamin A precursor in the early steps of muscle cell differentiation.
- Subjects :
- congenital, hereditary, and neonatal diseases and abnormalities
Time Factors
Caveolin 3
Cellular differentiation
Emerin
Biology
Muscle Development
Myoblasts
Mice
Animals
Humans
Protein Precursors
Muscle, Skeletal
Cells, Cultured
Cellular localization
integumentary system
Muscle cell differentiation
Gene Expression Regulation, Developmental
Membrane Proteins
Nuclear Proteins
Cell Differentiation
Cell Biology
Lamin Type A
Cell biology
DNA-Binding Proteins
Biochemistry
Nuclear lamina
Protein Processing, Post-Translational
C2C12
Lamin
Protein Binding
Subjects
Details
- ISSN :
- 00144827
- Volume :
- 314
- Database :
- OpenAIRE
- Journal :
- Experimental Cell Research
- Accession number :
- edsair.doi.dedup.....e28403b31121faef9eb6ddf43ad0d893