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Molecular characterization of avian polyomavirus isolated from psittacine birds based on the whole genome sequence analysis
- Source :
- Veterinary Microbiology. 138:69-77
- Publication Year :
- 2009
- Publisher :
- Elsevier BV, 2009.
-
Abstract
- Seven avian polyomaviruses (APVs) were isolated from seven psittacine birds of four species. Their whole genome sequences were genetically analyzed. Comparing with the sequence of BFDV1 strain, nucleotide substitutions in the sequences of seven APV isolates were found at 63 loci and a high level of conservation of amino acid sequence in each viral protein (VP1, VP2, VP3, VP4, and t/T antigen) was predicted. An A-to-T nucleotide substitution was observed in non-control region of all seven APV sequences in comparison with BFDV1 strain. Two C-to-T nucleotide substitutions were also detected in non-coding regions of one isolate. A phylogenetic analysis of the whole genome sequences indicated that the sequences from the same species of bird were closely related. APV has been reported to have distinct tropism for cell cultures of various avian species. The present study indicated that a single amino acid substitution at position 221 in VP2 was essential for propagating in chicken embryonic fibroblast culture and this substitution was promoted by propagation on budgerigar embryonic fibroblast culture. For two isolates, three serial amino acids appeared to be deleted in VP4. However, this deletion had little effect on virus propagation.
- Subjects :
- Male
Sequence analysis
viruses
Genome, Viral
Biology
Microbiology
Genome
Psittaciformes
Japan
Phylogenetics
Sequence Homology, Nucleic Acid
Animals
Amino Acid Sequence
RNA, Messenger
Melopsittacus
Peptide sequence
Conserved Sequence
Tropism
Mammals
Genetics
chemistry.chemical_classification
Polyomavirus Infections
Base Sequence
General Veterinary
Phylogenetic tree
Bird Diseases
Reverse Transcriptase Polymerase Chain Reaction
Nucleic acid sequence
virus diseases
General Medicine
Virology
Amino acid
chemistry
DNA, Viral
RNA, Viral
Female
Polyomavirus
Sequence Alignment
Subjects
Details
- ISSN :
- 03781135
- Volume :
- 138
- Database :
- OpenAIRE
- Journal :
- Veterinary Microbiology
- Accession number :
- edsair.doi.dedup.....e27913a8f34c24a875338ca6cec668c9