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Optimization of a magnetic bead-based assay (MAGPIX®-Luminex) for immune surveillance of exposure to malaria using multiple Plasmodium antigens and sera from different endemic settings
- Source :
- Malaria Journal, Malaria Journal, Vol 17, Iss 1, Pp 1-9 (2018), Malaria Journal, BioMed Central, 2018, 17 (1), pp.324. ⟨10.1186/s12936-018-2465-4⟩, Malaria Journal, 2018, 17 (1), pp.324. ⟨10.1186/s12936-018-2465-4⟩
- Publication Year :
- 2018
- Publisher :
- BioMed Central, 2018.
-
Abstract
- International audience; Background: Serological markers are potentially useful tools for monitoring the progress of malaria control programs , but a better understanding of antibody response dynamics is necessary. The use of a magnetic bead-based immunoassay (MBA) is advantageous compared to ELISA, due to its multiplexing capacity, but limited information is available on the standardization and validation of this assay. Methods: Several parameters for multiplex testing of antibodies to Plasmodium antigens were analysed using a set of 4 antigens and 98 sera from Senegalese rural asymptomatic and urban symptomatic individuals. The 4 antigens included Plasmodium falciparum CSP and PfAMA1 peptides, recombinant P. falciparum MSP4p20 and a Plasmodium malariae CSP (PmCSP) peptide. Comparisons with ELISA were done using MSP4p20 and whole schizont extract (SE) antigens.Results: The use of fewer beads (1000 beads per well instead of 2000) and 5 µg of antigen per 10 6 bead were validated as lower amounts. The use of a carrier protein (BSA) was shown to be critical when using peptides and the effect of a 24 h delayed measures was evaluated (5-25% signal decrease). Analysis of Ab responses showed almost equally high levels and prevalence in all transmission settings. Clear distinctions between rural and urban malaria were noted using PmCSP and SE antigens.Conclusions: This study underlines the importance of further optimization of the MBA technique and highlights the interest of using multistage/multispecies antigens for surveillance of malaria in endemic settings.
- Subjects :
- 0301 basic medicine
lcsh:Arctic medicine. Tropical medicine
lcsh:RC955-962
030231 tropical medicine
Plasmodium falciparum
Protozoan Proteins
Antigens, Protozoan
Plasmodium malariae
Biology
lcsh:Infectious and parasitic diseases
Serology
03 medical and health sciences
0302 clinical medicine
Antigen
parasitic diseases
medicine
lcsh:RC109-216
Multiplex
Malaria, Falciparum
IgG response
Immunoassay
medicine.diagnostic_test
Immunomagnetic Separation
Magnetic beads assay
Methodology
medicine.disease
biology.organism_classification
3. Good health
Malaria
030104 developmental biology
Infectious Diseases
Immunology
biology.protein
[SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie
Parasitology
Antibody
Biomarkers
Subjects
Details
- Language :
- English
- ISSN :
- 14752875
- Volume :
- 17
- Database :
- OpenAIRE
- Journal :
- Malaria Journal
- Accession number :
- edsair.doi.dedup.....e2706ecddc7a6dc1f82bcb69f58331e8