Back to Search
Start Over
Hyperfluidization-coupled membrane microdomain reorganization is linked to activation of the heat shock response in a murine melanoma cell line
- Source :
- Proceedings of the National Academy of Sciences. 104:7945-7950
- Publication Year :
- 2007
- Publisher :
- Proceedings of the National Academy of Sciences, 2007.
-
Abstract
- Targeting of the Hsp function in tumor cells is currently being assessed as potential anticancer therapy. An improved understanding of the molecular signals that trigger or attenuate the stress protein response is essential for advances to be made in this field. The present study provides evidence that the membrane fluidizer benzyl alcohol (BA), a documented nondenaturant, acts as a chaperone inducer in B16(F10) melanoma cells. It is demonstrated that this effect relies basically on heat shock transcription factor 1 (HSF1) activation. Under the conditions tested, the BA-induced Hsp response involves the up-regulation of a subset of hsp genes. It is shown that the same level of membrane fluidization (estimated in the core membrane region) attained with the closely analogous phenethyl alcohol (PhA) does not generate a stress protein signal. BA, at a concentration that activates heat shock genes, exerts a profound effect on the melting of raft-like cholesterol-sphingomyelin domains in vitro , whereas PhA, at a concentration equipotent with BA in membrane fluidization, has no such effect. Furthermore, through the in vivo labeling of melanoma cells with a fluorescein labeled probe that inserts into the cholesterol-rich membrane domains [fluorescein ester of polyethylene glycol-derivatized cholesterol (fPEG-Chol)], we found that, similarly to heat stress per se , BA, but not PhA, initiates profound alterations in the plasma membrane microdomain structure. We suggest that, apart from membrane hyperfluidization in the deep hydrophobic region, a distinct reorganization of cholesterol-rich microdomains may also be required for the generation and transmission of stress signals to activate hsp genes.
- Subjects :
- Hot Temperature
Membrane Fluidity
Lipid Bilayers
Melanoma, Experimental
Mice
Membrane Microdomains
Heat Shock Transcription Factors
Membrane region
Cell Line, Tumor
Membrane fluidity
Animals
HSP70 Heat-Shock Proteins
Heat shock
Promoter Regions, Genetic
HSF1
Lipid bilayer
Multidisciplinary
biology
Lipid microdomain
Phenylethyl Alcohol
Biological Sciences
Cell biology
DNA-Binding Proteins
Gene Expression Regulation, Neoplastic
Heat shock factor
Chaperone (protein)
biology.protein
Heat-Shock Response
Benzyl Alcohol
Transcription Factors
Subjects
Details
- ISSN :
- 10916490 and 00278424
- Volume :
- 104
- Database :
- OpenAIRE
- Journal :
- Proceedings of the National Academy of Sciences
- Accession number :
- edsair.doi.dedup.....e26e167e9ad042c5f25b33265238b494