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Screening, identification and validation of CCND1 and PECAM1/CD31 for predicting prognosis in renal cell carcinoma patients

Authors :
Feng Li
Rui Wang
Yunhua Qiu
Xiaoyun Song
Xiqiu Zhou
Jinzhou Zheng
Jianfeng Yang
Hailiang Zhang
Kui Yu
Shen-Nan Shi
Yuan-Yuan Qu
Wenhao Xu
Yu Wang
Source :
Aging (Albany NY)
Publication Year :
2019
Publisher :
Impact Journals, LLC, 2019.

Abstract

Clear cell renal cell carcinoma (ccRCC) is one of the most common cancers worldwide. Despite intense efforts to elucidate its pathogenesis, the molecular mechanisms and genetic characteristics of this cancer remain unknown. In this study, three expression profile data sets (GSE15641, GSE16441 and GSE66270) were integrated to identify candidate genes that could elucidate functional pathways in ccRCC. Expression data from 63 ccRCC tumors and 54 normal samples were pooled and analyzed. The GSE profiles shared 379 differentially expressed genes (DEGs), including 249 upregulated genes, and 130 downregulated genes. A protein-protein interaction network (PPI) was constructed and analyzed using STRING and Cytoscape. Functional and signaling pathways of the shared DEGs with significant p values were identified. Kaplan-Meier plots of integrated expression scores were used to analyze survival outcomes. These suggested that FN1, ICAM1, CXCR4, TYROBP, EGF, CAV1, CCND1 and PECAM1/CD31 were independent prognostic factors in ccRCC. Finally, to investigate early events in renal cancer, we screened for the hub genes CCND1 and PECAM1/CD31. In summary, integrated bioinformatics analysis identified candidate DEGs and pathways in ccRCC that could improve our understanding of the causes and underlying molecular events of ccRCC. These candidate genes and pathways could be therapeutic targets for ccRCC.

Details

ISSN :
19454589
Volume :
11
Database :
OpenAIRE
Journal :
Aging
Accession number :
edsair.doi.dedup.....e26c2be4dee4e5fefb0205e4724ccc76