MOESM1 of How does cellulosome composition influence deconstruction of lignocellulosic substrates in Clostridium (Ruminiclostridium) thermocellum DSM 1313?

Additional file 1: Figure S1. Purification profile of the different cellulosomes by Gel filtration chromatography. C. thermocellum growth media were centrifuged (10,900Â g, 7Â min), and the supernatant fluids were carefully removed from the pellet and concentrated 40 times using a Pellicon XL biomax 300 cassette (Millipore, Cat. No. PXB300C50). Concentrated samples were fractionated by size exclusion chromatography using a SuperdexS-200 prep grade 16/60 gel filtration column (GE Healthcare). (A) Chromatogram of glucose-derived cellulosomes (B) Chromatogram of CB-derived cellulosomes (C) Chromatogram of MCC-derived cellulosomes (D) Chromatogram of alSG-derived cellulosomes (E) Chromatogram of alCS-derived cellulosomes (F) Chromatogram of acCS-derived cellulosomes. Fractions containing the cellulosomes (according to SDS-PAGE analysis) are marked by black arrows. All cellulosomes-containing fractions were eluted immediately after void value due to the separation range of the column.