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Effect of the Combination of Levofloxacin with Cationic Carbosilane Dendron and Peptide in the Prevention and Treatment of Staphylococcus aureus Biofilms
- Source :
- Polymers, Volume 13, Issue 13, Polymers, Vol 13, Iss 2127, p 2127 (2021)
- Publication Year :
- 2021
- Publisher :
- Multidisciplinary Digital Publishing Institute, 2021.
-
Abstract
- Antibiotic resistance and biofilm-related infections, persistent in conventional antimicrobial treatment, are continuously increasing and represent a major health problem worldwide. Therefore, the development of new effective treatments to prevent and treat biofilm-related infections represents a crucial challenge. Unfortunately, the extensive use of antibiotics has led to an increase of resistant bacteria with the subsequent loss of effectivity of commercial antibiotics, mainly due to antibiotic resistance and the ability of some bacteria to form microbial communities in biotic or abiotic surfaces (biofilms). In some cases, these biofilms are resistant to high concentrations of antibiotics that lead to treatment failure and recurrence of the associated infections. In the fight against microbial resistance, the combination of traditional antibiotics with new compounds (combination therapy) is an alternative that is becoming more extensive in the medical field. In this work, we studied the cooperative effects between levofloxacin, an approved antibiotic, and peptides or cationic dendritic molecules, compounds that are emerging as a feasible solution to overcome the problem of microbial resistance caused by pathogenic biofilms. We studied a new therapeutic approach that involves the use of levofloxacin in combination with a cationic carbosilane dendron, called MalG2(SNHMe2Cl)4, or a synthetic cell-penetrating peptide, called gH625, conjugated to the aforementioned dendron. To carry out the study, we used two combinations (1) levofloxacin/dendron and (2) levofloxacin/dendron-peptide nanoconjugate. The results showed the synergistic effect of the combination therapy to treat Staphylococcus aureus biofilms. In addition, we generated a fluorescein labeled peptide that allowed us to observe the conjugate (dendron-peptide) localization throughout the bacterial biofilm by confocal laser scanning microscopy.
- Subjects :
- Staphylococcus aureus
Polymers and Plastics
Combination therapy
medicine.drug_class
Antibiotics
dendron
Organic chemistry
medicine.disease_cause
Article
biofilm
Microbiology
03 medical and health sciences
QD241-441
Antibiotic resistance
Levofloxacin
medicine
030304 developmental biology
0303 health sciences
biology
030306 microbiology
Chemistry
Biofilm
General Chemistry
biochemical phenomena, metabolism, and nutrition
biology.organism_classification
Antimicrobial
peptide
Staphylococcus aureu
nanoconjugate
Bacteria
medicine.drug
Subjects
Details
- Language :
- English
- ISSN :
- 20734360
- Database :
- OpenAIRE
- Journal :
- Polymers
- Accession number :
- edsair.doi.dedup.....e25aa37efb5b690bd6a448ffd94d2458
- Full Text :
- https://doi.org/10.3390/polym13132127