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Determination of Biogenic Amines in Different Parts of Lycium barbarum L. by HPLC with Precolumn Dansylation
- Source :
- Molecules, Volume 26, Issue 4, Molecules, Vol 26, Iss 1046, p 1046 (2021)
- Publication Year :
- 2021
- Publisher :
- Multidisciplinary Digital Publishing Institute, 2021.
-
Abstract
- The aim of this work was to characterize biogenic amines (BAs) in different parts of Lycium barbarum L. using HPLC with dansyl chloride derivatization, and jointly, to provide referential data for further exploration and utilization of Lycium barbarum L. The linear correlation coefficients for all BAs were above 0.9989. The limits of detection and quantification were 0.015–0.075 and 0.05–0.25 μg/mL, respectively. The relative standard deviations for the intra-day and inter-day precision were 0.66–2.69% and 0.91–4.38%. The described method has good repeatability and intermediate precision for the quantitative determination of BAs in different parts of Lycium barbarum L. Satisfactory recovery for all amines was obtained (79.3–110.3%). The result showed that there were four kinds of BAs. The highest putrescine content (20.9 ± 3.2 mg/kg) was found in the flower. The highest histamine content (102.7 ± 5.8 mg/kg) was detected in the bark, and the highest spermidine (13.3 ± 1.6 mg/kg) and spermine (23.7 ± 2.0 mg/kg) contents were detected in the young leaves. The high histamine (HIS) content in the bark may be one of the reasons why all of the parts of Lycium barbarum L., except the bark, are used for medicine or food in China. Meanwhile, the issue of the high concentration of HIS should be considered when exploiting or utilizing the bark of Lycium barbarum L.
- Subjects :
- Pharmaceutical Science
biogenic amines
01 natural sciences
High-performance liquid chromatography
Mass Spectrometry
Article
Analytical Chemistry
lcsh:QD241-441
chemistry.chemical_compound
0404 agricultural biotechnology
lcsh:Organic chemistry
Lycium barbarum L
Drug Discovery
derivatization
Physical and Theoretical Chemistry
Derivatization
Chromatography, High Pressure Liquid
Dansyl Compounds
Detection limit
Chromatography
biology
Chemistry
010401 analytical chemistry
Organic Chemistry
Dansyl chloride
Reproducibility of Results
04 agricultural and veterinary sciences
Lycium
Reference Standards
biology.organism_classification
040401 food science
0104 chemical sciences
Solutions
Spermidine
Chemistry (miscellaneous)
visual_art
visual_art.visual_art_medium
Putrescine
Molecular Medicine
Bark
HPLC
Subjects
Details
- Language :
- English
- ISSN :
- 14203049
- Database :
- OpenAIRE
- Journal :
- Molecules
- Accession number :
- edsair.doi.dedup.....e223c4a4606141a532bbff2d4a9841b8
- Full Text :
- https://doi.org/10.3390/molecules26041046