Back to Search
Start Over
Alteration of cell population structure due to cell lysis inSaccharomyces cerevisiae cells overexpressing theGAL4 gene
- Source :
- Yeast. 9:575-582
- Publication Year :
- 1993
- Publisher :
- Wiley, 1993.
-
Abstract
- Transformed Saccharomyces cerevisiae cells overexpressing the Escherichia coli LacZ gene and the transcriptional activator GAL4, release in the external medium a fraction (from 2 to 10%) of the total β-galactosidase activity (Porro et al., 1992b). It is known that this abnormal release of a cytoplasmic protein is related to a partial cell lysis of the yeast population, which is likely to be caused by the overexpression of the transcriptional activator GAL4. In the present paper we have characterized the GAL4-induced cell lysis phenomenon. The expression of the GAL4 gene causes morphological modifications and alteration of the cell size distribution. The cell lysis is independent of the expression of the heterologous LacZ gene and occurs in a specific subpopulation of cells (the parent cells) independently of the genealogical age, growth phase conditions and cell cycle progression. Lysis is preceded by a loss of the plasma membrane integrity as indicated by the uptake of ethidium bromide in unfixed cells. Computer analysis of simulated protein distributions indicates that cell lysis takes place in a sizeable aliquot (about 50%) of the parent cells, therefore profoundly altering the age structure of the population.
- Subjects :
- Saccharomyces cerevisiae Proteins
Lysis
Transcription Factor
DNA-Binding Protein
Genes, Fungal
Cell
Population
Saccharomyces cerevisiae
Fungal Protein
lac operon
Bioengineering
Biology
Cell Fractionation
Models, Biological
Applied Microbiology and Biotechnology
Biochemistry
Flow cytometry
Fungal Proteins
Transformation, Genetic
Gene Expression Regulation, Fungal
Genetics
medicine
education
education.field_of_study
medicine.diagnostic_test
Flow Cytometry
beta-Galactosidase
biology.organism_classification
Molecular biology
DNA-Binding Proteins
Cytolysis
medicine.anatomical_structure
Cell fractionation
Saccharomyces cerevisiae Protein
Transcription Factors
Biotechnology
Subjects
Details
- ISSN :
- 10970061 and 0749503X
- Volume :
- 9
- Database :
- OpenAIRE
- Journal :
- Yeast
- Accession number :
- edsair.doi.dedup.....e21470db676abebb6d5e24ad2b942621