Back to Search
Start Over
Isolation and characterization of enantioselective DNA aptamers for ibuprofen
- Source :
- Bioorganicmedicinal chemistry. 18(10)
- Publication Year :
- 2010
-
Abstract
- Single stranded DNA aptamers that can bind to ibuprofen, a widely used anti-inflammation drug, were selected from random DNA library of 10 15 nucleotides by FluMag-SELEX process. Five different sequences were selected and their enantioselectivity and affinity were characterized. Three out of five aptamer candidates did not show any affinity to ( S )-ibuprofen, but only to racemic form of ibuprofen, suggesting that they are ( R )-ibuprofen specific aptamers. Another two aptamer candidates showed affinity to both racemic form and ( S )-ibuprofen, which were considered as ( S )-ibuprofen specific aptamers. The affinity of five ssDNA aptamers isolated was in a range of 1.5–5.2 μM. In addition, all of these five aptamers did not show any affinity to analogues of ibuprofen in its profen’s group (fenoprofen, flubiprofen, and naproxen) and the antibiotics of oxytetracycline, another control.
- Subjects :
- DNA, Bacterial
Naproxen
Stereochemistry
Aptamer
Clinical Biochemistry
Molecular Sequence Data
Oligonucleotides
Pharmaceutical Science
DNA, Single-Stranded
Ibuprofen
DNA Aptamers
Biochemistry
Structure-Activity Relationship
Drug Discovery
medicine
Nucleotide
Molecular Biology
Gene Library
chemistry.chemical_classification
Fenoprofen
Binding Sites
Base Sequence
organic chemicals
Organic Chemistry
SELEX Aptamer Technique
Enantioselective synthesis
Aptamers, Nucleotide
Kinetics
chemistry
Molecular Medicine
Nucleic Acid Conformation
Systematic evolution of ligands by exponential enrichment
medicine.drug
Subjects
Details
- ISSN :
- 14643391
- Volume :
- 18
- Issue :
- 10
- Database :
- OpenAIRE
- Journal :
- Bioorganicmedicinal chemistry
- Accession number :
- edsair.doi.dedup.....e20647bb0a97badf9c23fd9b4cef2d39