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The effect of dietary nitrate supplementation on the speed-duration relationship in mice with sickle cell disease
- Source :
- J Appl Physiol (1985)
- Publication Year :
- 2020
-
Abstract
- Sickle cell disease (SCD) causes exercise intolerance likely due to impaired skeletal muscle function and low nitric oxide (NO) bioavailability. Dietary nitrate improves hemodynamic and metabolic control during exercise in humans and animals. The purpose of this investigation was to assess the impact of nitrate supplementation on exercise capacity as measured by the running speed to exercise duration relationship [critical speed (CS)]in mice with SCD. We tested the hypothesis that nitrate supplementation via beetroot juice (BR) would attenuate the exercise intolerance observed in mice with SCD. Ten wild-type (WT) and 18 Berkley sickle-cell mice (BERK) received water (WT: n = 10, BERK: n = 10) or nitrate-rich BR (BERK+BR: n = 8, nitrate dose 1 mmol/kg/day) for 5 days. Following the supplementation period, all mice performed 3–5 constant-speed treadmill tests that resulted in exhaustion within 1.5 to 20 min. Time to exhaustion vs. treadmill speed was fit to a hyperbolic model to determine CS. CS was significantly lower in BERK vs. WT and BERK+BR with no significant difference between WT and BERK+BR (WT: 36.6 ± 1.6, BERK: 23.8 ± 1.5, BERK+BR: 31.1 ± 2.1 m/min, P < 0.05). Exercise tolerance, measured via CS, was significantly lower in BERK mice relative to WT. However, BERK mice receiving 5 days of nitrate supplementation exhibited no difference in exercise tolerance when compared with WT. These results support the potential utility of a dietary nitrate intervention to improve functionality in SCD patients. NEW & NOTEWORTHY Sickle cell disease compromises muscle O(2) delivery resulting in exercise intolerance. Dietary nitrate supplementation increases skeletal muscle blood flow during exercise and may improve exercise capacity in a mouse model of sickle cell disease. We investigated the effects of dietary nitrate supplementation on exercise tolerance in a mouse model of sickle cell disease using the treadmill speed-duration relationship (critical speed). Mice with sickle cell disease provided with a dietary nitrate supplement had a critical speed not significantly different from healthy wild-type mice.
- Subjects :
- medicine.medical_specialty
Physiology
Cell
Exercise intolerance
Disease
Anemia, Sickle Cell
030204 cardiovascular system & hematology
Nitric oxide
03 medical and health sciences
chemistry.chemical_compound
Mice
0302 clinical medicine
Oxygen Consumption
Double-Blind Method
Physiology (medical)
Internal medicine
Dietary Nitrate
medicine
Animals
Humans
Exercise Tolerance
Nitrates
business.industry
medicine.anatomical_structure
Endocrinology
chemistry
Dietary Supplements
Skeletal muscle blood flow
Free hemoglobin
medicine.symptom
Beta vulgaris
business
030217 neurology & neurosurgery
Research Article
Subjects
Details
- ISSN :
- 15221601
- Volume :
- 129
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Journal of applied physiology (Bethesda, Md. : 1985)
- Accession number :
- edsair.doi.dedup.....e202517438a8b80d7ad2e7bc20cade98