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Molecular characterization of Campylobacter jejuni from patients with Guillain-Barré and Miller Fisher syndromes
- Source :
- Journal of Clinical Microbiology 38 (2000), Journal of Clinical Microbiology, 38, 2297-2301, Journal of Clinical Microbiology, 38(6), 2297-2301. American Society for Microbiology
- Publication Year :
- 2000
-
Abstract
- Campylobacter jejuni has been identified as the predominant cause of antecedent infection in Guillain-Barrésyndrome (GBS) and Miller Fisher syndrome (MFS). The risk of developing GBS or MFS may be higher after infection with specific C. jejuni types. To investigate the putative clonality, 18 GBS- or MFS-related C. jejuni strains from The Netherlands and Belgium and 17 control strains were analyzed by serotyping (Penner and Lior), restriction fragment length polymorphism analysis of PCR products of the flaA gene, amplified fragment length polymorphism analysis, pulsed-field gel electrophoresis, and randomly amplified polymorphic DNA analysis. Serotyping revealed 10 different O serotypes and 7 different Lior serotypes, thereby indicating a lack of serotype clustering. Two new O serotypes, O:35 and O:13/65, not previously associated with GBS or MFS were found. Serotype O:19 was encountered in 2 of 18 strains, and none was of serotype O:41. The results of all genotypic methods also demonstrated substantial heterogeneity. No clustering of GBS- or MFS-related strains occurred and no molecular marker capable of separating pathogenic GBS or MFS from non-GBS- or non-MFS-related enteritis strains could be identified in this study. Sialic-acid-containing lipopolysaccharides (LPS) are thought to be involved in the triggering of GBS or MFS through molecular mimicry with gangliosides in human peripheral nerves. Therefore, further characterization of GBS- or MFS-related C. jejuni should target the genes involved in the synthesis of LPS and the incorporation of sialic acid.
- Subjects :
- Microbiology (medical)
Serotype
Genotype
Biology
Guillain-Barre Syndrome
Campylobacter jejuni
Microbiology
law.invention
Enteritis
law
medicine
Life Science
Humans
Serotyping
Polymerase chain reaction
Amplified Fragment Length Polymorphism Analysis
Miller Fisher Syndrome
Molecular epidemiology
Genetic Variation
Bacteriology
medicine.disease
biology.organism_classification
bacterial infections and mycoses
DNA Fingerprinting
Bacterial Typing Techniques
Electrophoresis, Gel, Pulsed-Field
Random Amplified Polymorphic DNA Technique
ID-Lelystad, Instituut voor Dierhouderij en Diergezondheid
ID Lelystad, Institute for Animal Science and Health
Restriction fragment length polymorphism
Polymorphism, Restriction Fragment Length
Flagellin
Subjects
Details
- ISSN :
- 00951137
- Volume :
- 38
- Issue :
- 6
- Database :
- OpenAIRE
- Journal :
- Journal of clinical microbiology
- Accession number :
- edsair.doi.dedup.....e1fc46cb4e736cb294bc484344939fce