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Pediatric Acute Lymphoblastic Leukemia Patients Exhibit Distinctive Alterations in the Gut Microbiota
- Source :
- Frontiers in Cellular and Infection Microbiology, Frontiers in Cellular and Infection Microbiology, Vol 10 (2020)
- Publication Year :
- 2020
- Publisher :
- Frontiers Media S.A., 2020.
-
Abstract
- Previous studies have shown that gut microbiota can affect human immune system in many ways. Our aim was to investigate quantitative differences in fecal bacterial compositions of childhood acute lymphoblastic leukemia (ALL) patients compared to those of healthy children, so as to identify individual bacterial species that are related to the etiology of ALL. We recruited 81 subjects, including 58 patients with ALL and 23 healthy controls. Fecal samples were collected and examined by 16S rRNA quantitative arrays and bioinformatics analysis. Both Principal Coordinates Analysis (PCoA) and Non-metric Multidimensional scaling (NMDS) demonstrated that the microbial composition of ALL patients deviated from the tight cluster of healthy controls. Multiple bacterial species exhibited significant changes (e.g., Roseburia faecis, Edwardsiella tarda, and Fusobacterium naviforme) in the ALL samples. Some of the differentially abundant taxa were correlated with the level of interleukin-10. The ALL cases could be efficiently distinguished from healthy controls by the random forest model based on differential species (area under ROC curve = 0.843). Taken together, the composition of gut microbiota differed from healthy controls to pediatric ALL patients. Our study identified a series of ALL-related species in the gut microbiota, providing a new direction for future studies aiming to understand the host-gut microbiota interplay in ALL pathogenesis.
- Subjects :
- Microbiology (medical)
Immunology
interleukin-10
lcsh:QR1-502
Gut flora
Microbiology
lcsh:Microbiology
Pathogenesis
Immune system
Cellular and Infection Microbiology
acute lymphoblastic leukemia (ALL)
RNA, Ribosomal, 16S
Humans
Child
Childhood Acute Lymphoblastic Leukemia
Feces
Original Research
Clostridiales
biology
gut microbiota
Edwardsiella tarda
16S rRNA quantitative microarray
Fusobacterium
Precursor Cell Lymphoblastic Leukemia-Lymphoma
biology.organism_classification
Gastrointestinal Microbiome
Infectious Diseases
Fusobacterium naviforme
quantitative PCR
Etiology
Subjects
Details
- Language :
- English
- ISSN :
- 22352988
- Volume :
- 10
- Database :
- OpenAIRE
- Journal :
- Frontiers in Cellular and Infection Microbiology
- Accession number :
- edsair.doi.dedup.....e1e0f0608422ae396df1777c6a6814e2