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Incorporation of N-acetyl glucosamine into lipid linked oligosaccharides
- Source :
- Biochemical and Biophysical Research Communications. 70:1014-1019
- Publication Year :
- 1976
- Publisher :
- Elsevier BV, 1976.
-
Abstract
- When beef liver microsomes are labeled with UDP-[3H]N-acetyl glucosamine, three different lipid-bound saccharides are labeled: dolichol pyrophosphoryl-GlcNAc, dolichol pyrophosphoryl-(GlcNAc)2 and a previously uncharacterized compound (component III). Incubation with UDP leads to the disappearance of dolichol pyrophosphoryl-(GlcNac)2 from microsomes prelabeled with UDP-3H-N-acetyl glucosamine. This result provides further support for the suggestion of Leloir et al. (6) that dolichol pyrophosphoryl-(GlcNAc)2 is synthesized from dolichol pyrophosphoryl-GlcNAc and UDP-N-acetyl glucosamine. Component III cannot be discharged with UMP or UDP. It elutes from DEAE-cellulose exactly as the other components. The saccharide portion of component III has a molecular weight of 800–900 and contains glucose in addition to N-acetyl glucosamine.
- Subjects :
- Glucosamine
Uridine Diphosphate N-Acetylglucosamine
Lipid linked oligosaccharide
Biophysics
Oligosaccharides
Cell Biology
Biochemistry
Acetylglucosamine
carbohydrates (lipids)
chemistry.chemical_compound
Dolichol
Uridine diphosphate N-acetylglucosamine
Glycolipid
chemistry
Microsomes, Liver
Microsome
Animals
Cattle
lipids (amino acids, peptides, and proteins)
N acetyl glucosamine
Glycolipids
Molecular Biology
Subjects
Details
- ISSN :
- 0006291X
- Volume :
- 70
- Database :
- OpenAIRE
- Journal :
- Biochemical and Biophysical Research Communications
- Accession number :
- edsair.doi.dedup.....e1d668efdbe33a74008b69113c0890ed