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Adhesion to and invasion of cultured tick (Acarina: Ixodidae) cells by Borrelia burgdorferi (Spirochaetales: Spirochaetaceae) and maintenance of infectivity
- Source :
- Journal of medical entomology. 30(3)
- Publication Year :
- 1993
-
Abstract
- Lyme disease spirochetes, Borrelia burgdorferi, interact with cultured tick cells in ways similar to those reported to occur in the vector Ixodes dammini Spielman, Clifford, Piesman & Corwin. Spirochete adhesion and penetration were examined using a cell line from embryos of Rhipicephalus appendiculatus Neumann that morphologically resembles tick gut cells, RAE25. Cocultivation of B. burgdorferi with these cells permitted prolonged maintenance of infectivity for hamsters. Borrelial adherence to RAE25 cells was time- and density-dependent and increased by 10—15% per h during the first 5.5 h of cocultivation when we used a concentration of 4 × 107 spirochetes/ml. After 6 h, >90% of the cells bound an average of 3*.xml5 spirochetes per cell. Low passage, hamster-infective strains of B. burgdorferi (JMNT and CD16) showed a 2-3-fold higher rate of adhesion to RAE25 cells than the highly passaged, noninfectious strain B31. Inactivation of CD16 or JMNT by heat, starvation, or treatment with puromycin reduced adherence by 40*.xml60%, whereas preheatment with monoclonal antibodies to the outer surface proteins had no effect. Spirochetes adhered to young I. dammini cell lines to a similar degree as they did to RAE25, whereas lines from the ticks Dermacentor variabilis (Say) (RML15) and Boophilus microplus (Canestrini) (BME26) bound 30—60% fewer spirochetes. Electron microscopy revealed epicellular borreliae associated with coated pits and vesicles before endocytosis, and intracellular spirochetes were surrounded by a host cell-derived membrane.
- Subjects :
- Infectivity
General Veterinary
biology
Spirochaetaceae
Tick
biology.organism_classification
medicine.disease
Bacterial Adhesion
Microbiology
Cell Line
Microscopy, Electron
Infectious Diseases
Lyme disease
Ticks
Borrelia burgdorferi Group
Cell culture
Insect Science
Cricetinae
parasitic diseases
medicine
Animals
Parasitology
Borrelia burgdorferi
Dermacentor variabilis
Ixodidae
Subjects
Details
- ISSN :
- 00222585
- Volume :
- 30
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Journal of medical entomology
- Accession number :
- edsair.doi.dedup.....e1c72478d14ce190b903e048ef3a0908