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Peripheral blood vessels are a niche for blood-borne meningococci
- Source :
- Virulence, Virulence, 2017, 8 (8), pp.1808-1819. ⟨10.1080/21505594.2017.1391446⟩, Virulence, 8(8), 1808. Landes Bioscience
- Publication Year :
- 2017
-
Abstract
- International audience; Neisseria meningitidis is the causative agent of cerebrospinal meningitis and that of a rapidly progressing fatal septic shock known as purpura fulminans. Meningococcemia is characterized by bacterial adhesion to human endothelial cells of the microvessels. Host specificity has hampered studies on the role of blood vessels colonization in N. meningitidis associated pathogenesis. In this work, using a humanized model of SCID mice allowing the study of bacterial adhesion to human cells in an in vivo context we demonstrate that meningococcal colonization of human blood vessels is a prerequisite to the establishment of sepsis and lethality. To identify the molecular pathways involved in bacterial virulence, we performed transposon insertion site sequencing (Tn-seq) in vivo. Our results demonstrate that 36% of the genes that are important for growth in the blood of mice are dispensable when bacteria colonize human blood vessels, suggesting that human endothelial cells lining the blood vessels are feeding niches for N. meningitidis in vivo. Altogether, our work proposes a new paradigm for meningococcal virulence in which colonization of blood vessels is associated with metabolic adaptation and sustained bacteremia responsible for sepsis and subsequent lethality.
- Subjects :
- 0301 basic medicine
Microbiology (medical)
Immunology
Virulence
Context (language use)
Bacteremia
Mice, SCID
Biology
Neisseria meningitidis
medicine.disease_cause
purpura fulminans
Microbiology
Bacterial Adhesion
Sepsis
03 medical and health sciences
nutritional virulence
Mice
Bacterial Proteins
In vivo
[SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases
medicine
Animals
Humans
[SDV.MP] Life Sciences [q-bio]/Microbiology and Parasitology
Mice, Inbred BALB C
Septic shock
host cell interaction
Tn-seq
medicine.disease
[SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology
Meningococcal Infections
030104 developmental biology
Infectious Diseases
[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology
Editorial
Microvessels
[SDV.MHEP.MI] Life Sciences [q-bio]/Human health and pathology/Infectious diseases
Parasitology
Female
[SDV.MP.BAC] Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology
Purpura fulminans
Subjects
Details
- ISSN :
- 21505608 and 21505594
- Volume :
- 8
- Issue :
- 8
- Database :
- OpenAIRE
- Journal :
- Virulence
- Accession number :
- edsair.doi.dedup.....e1beb6a32171c77300559b57107b9cb7