Pseudochrobactrum algeriensis sp. nov., isolated from lymph nodes of Algerian cattle

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Three Gram-negative, rod-shaped, oxidase-positive, non-spore-forming, non-motile strains (C130915_07T, C150915_16 and C150915_17) were isolated from lymph nodes of Algerian cows. On the basis of 16S rRNA gene and whole genome similarities, the isolates were almost identical and clearly grouped in the genus Pseudochrobactrum . This allocation was confirmed by the analysis of fatty acids (C19:cyclo, C18 : 1, C18 : 0, C16 : 1 and C16 : 0) and of polar lipids (major components: phosphatidylethanolamine, ornithine-lipids, phosphatidylglycerol, cardiolipin and phosphatidylcholine, plus moderate amounts of phosphatidylmonomethylethanolamine, phosphatidyldimethylethanolamine and other aminolipids). Genomic, physiological and biochemical data differentiated these isolates from previously described Pseudochrobactrum species in DNA relatedness, carbon assimilation pattern and growth temperature range. Thus, these organisms represent a novel species of the genus Pseudochrobactrum , for which the name Pseudochrobactrum algeriensis sp. nov. is proposed (type strain C130915_07T=CECT30232T=LMG 32378T).
Research at the University of Navarra was supported by the ISTUN Institute of Tropical Health, University of Navarra Health funders (Fundación la CAIXA -LCF/PR/PR13/11080005) and Fundación Caja Navarra, Fundación María Francisca de Roviralta, Ubesol and Inversiones Garcilaso de la Vega S.L) and MINECO grants AGL2014-58795-C4-1-R (MINECO/AEI/FEDER) and PID2019-107601RA-C32 (MCIN/AEI/ 10.1303910.13039/501100011033). M.L.-B. is recipient of the PhD. Fellowships Formación de Profesorado Universitario (FPU) funded by Ministerio de Ciencia, Innovación y Universidad (Spanish Government) and Ayuda predoctoral from Gobierno de Navarra. Work at INRAE was supported by Agence Nationale de la Recherche Grant ASTRID-Maturation ANR-14-ASMA-0002-02. Work at IRNAS was supported by Consejo Superior de Investigaciones Científicas (Grant 202040E185).