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Measurement of cholesterol and triglycerides from a dried blood spot in an Indian Council of Medical Research-World Health Organization multicentric survey on risk factors for noncommunicable diseases in India
- Source :
- Journal of clinical lipidology. 6(1)
- Publication Year :
- 2011
-
Abstract
- Background Dried blood may be a convenient method of sample collection in epidemiological studies; however, the method needs evaluation in a field settings. In the present study, feasibility of using dried blood for measurement of cholesterol and triglycerides was evaluated in multicenter surveillance study for noncommunicable disease (NCD). Methods Samples were collected in a cross-sectional study for NCD risk factor surveillance conducted in six centers in India. For every tenth subject recruited, a blood sample was also collected on filter paper. These 10% serum samples and dried blood spots were analyzed for cholesterol and triglycerides. Results The mean coefficient of variation (CV) for cholesterol was less than 10% between dried blood and serum in five of the six participating centers. Only one center showed a high CV of 14%. Similarly, the mean bias was less than 10% in five centers. The intraclass correlation between cholesterol values in dried blood and serum were greater than 0.638 in all centers, which suggests a good homogeneity of results. The mean CV for triglycerides ranged from 0.36% to 17.97%. The intraclass correlation between triglyceride values in dried blood and serum ranged from 0.756 to 0.880 in the six centers. Conclusion In conclusion, dried blood would be a good method for collection of blood for measurement of cholesterol and triglycerides for population health surveys. However, the benefits of blood spot analysis should be weighed against potential sources of errors attributable to sampling and other factors, such as temperature and humidity, in a country like India.
- Subjects :
- Adult
Male
medicine.medical_specialty
Veterinary medicine
Adolescent
Cross-sectional study
Intraclass correlation
Endocrinology, Diabetes and Metabolism
Coronary Artery Disease
World Health Organization
chemistry.chemical_compound
Young Adult
Risk Factors
Diabetes mellitus
Epidemiology
Internal Medicine
medicine
Humans
Risk factor
Triglycerides
Nutrition and Dietetics
Triglyceride
business.industry
Middle Aged
medicine.disease
Surgery
Dried blood spot
Cholesterol
Cross-Sectional Studies
chemistry
Feasibility Studies
Female
Sample collection
Dried Blood Spot Testing
Cardiology and Cardiovascular Medicine
business
Subjects
Details
- ISSN :
- 19332874
- Volume :
- 6
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Journal of clinical lipidology
- Accession number :
- edsair.doi.dedup.....e19db88fd7ce0c94752402bbfa833755