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Cloning and expression of the sucrose phosphorylase gene from Leuconostoc mesenteroides in Escherichia coli
- Source :
- Biotechnology Letters. 30:749-754
- Publication Year :
- 2007
- Publisher :
- Springer Science and Business Media LLC, 2007.
-
Abstract
- The gene encoding sucrose phosphorylase (742sp) in Leuconostoc mesenteroides NRRL B-742 was cloned and expressed in Escherichia coli. The nucleotide sequence of the transformed 742sp comprised an ORF of 1,458 bp giving a protein with calculated molecular mass of 55.3 kDa. 742SPase contains a C-terminal amino acid sequence that is significantly different from those of other Leu. mesenteroides SPases. The purified 742SPase had a specific activity of 1.8 U/mg with a K (m) of 3 mM with sucrose as a substrate; optimum activity was at 37 degrees C and pH 6.7. The purified 742SPase transferred the glucosyl moiety of sucrose to cytosine monophosphate (CMP).
- Subjects :
- Cytidine monophosphate
Sucrose
Molecular Sequence Data
Gene Expression
Bioengineering
Biology
medicine.disease_cause
Applied Microbiology and Biotechnology
Substrate Specificity
chemistry.chemical_compound
Bacterial Proteins
Cytidine Monophosphate
Escherichia coli
medicine
Leuconostoc
Amino Acid Sequence
Cloning, Molecular
Peptide sequence
Molecular Structure
Sequence Homology, Amino Acid
Molecular mass
Nucleic acid sequence
Sucrose phosphorylase
General Medicine
biology.organism_classification
Molecular biology
Recombinant Proteins
carbohydrates (lipids)
chemistry
Biochemistry
Glucosyltransferases
Leuconostoc mesenteroides
Electrophoresis, Polyacrylamide Gel
Chromatography, Thin Layer
Biotechnology
Subjects
Details
- ISSN :
- 15736776 and 01415492
- Volume :
- 30
- Database :
- OpenAIRE
- Journal :
- Biotechnology Letters
- Accession number :
- edsair.doi.dedup.....e19b6c4dbe53f935756356c98a4bf94d