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Initial measurement of the intracellular glass transition temperature of a mammalian cell type under cryopreservation conditions
Initial measurement of the intracellular glass transition temperature of a mammalian cell type under cryopreservation conditions
- Source :
- Cryobiology, 52nd Annual Meeting of the Society for Crryobiology, 52nd Annual Meeting of the Society for Crryobiology, Jul 2015, Ostrava, Czech Republic. pp.538, ⟨10.1016/j.cryobiol.2015.10.008⟩
- Publication Year :
- 2015
- Publisher :
- Elsevier BV, 2015.
-
Abstract
- A key physical event during the cooling of cells is the temperature at which the intracellular compartment undergoes vitrification. Recently (http://dx. doi.org/10.1371/journal.pone.0066207) we have measured the intracellular glass transition temperature (Tg) of bacteria using differential scanning calorimetry (DSC). Here we report the first data on the intracellular Tg of a mammalian cell cooled with and without a cryoprotectant (Me2SO). Jurkat cells (an immortalized T cell line) were concentrated and a pellet of approximately 30 mg cooled in a DSC, SnowMax was added to induce ice nucleation. On warming an intracellular glass transition was detected by the deviation in the first derivative of the heat flow, associated with a change in heat capacity as the cells devitrify at Tg. The transition temperature measured by DSC is Tg', that is the Tg of the maximally freezeconcentrated intracellular matrix. In the presence of Me2SO intracellular Tg was measured to be - 40 C, in controls (no Me2SO) Tg occurred at higher temperatures. In further experiments the temperature of the lipid phase transitions were analyzed by FTIR, and were measured to occur above freezing temperatures. The implications of the measured values of Tg’ for the basic cryobiology of mammalian cells will be discussed.
- Subjects :
- Phase transition
030219 obstetrics & reproductive medicine
Cryobiology
Chemistry
[SDV]Life Sciences [q-bio]
Transition temperature
cryopreservation condition
0402 animal and dairy science
04 agricultural and veterinary sciences
General Medicine
040201 dairy & animal science
Heat capacity
General Biochemistry, Genetics and Molecular Biology
mammalian cell type
Cell biology
intracellular glass transition
03 medical and health sciences
0302 clinical medicine
Differential scanning calorimetry
13. Climate action
Biophysics
Vitrification
General Agricultural and Biological Sciences
Glass transition
Intracellular
Subjects
Details
- ISSN :
- 00112240
- Volume :
- 71
- Database :
- OpenAIRE
- Journal :
- Cryobiology
- Accession number :
- edsair.doi.dedup.....e1930e4ece4f49c21fd688cdb50b4fee
- Full Text :
- https://doi.org/10.1016/j.cryobiol.2015.10.008