Back to Search
Start Over
An Optimized and Standardized Rapid Flow Cytometry Functional Method for Heparin-Induced Thrombocytopenia
- Source :
- Biomedicines, Volume 9, Issue 3, Biomedicines, Vol 9, Iss 296, p 296 (2021)
- Publication Year :
- 2021
- Publisher :
- MDPI, 2021.
-
Abstract
- Heparin-induced thrombocytopenia (HIT) is a thrombocytopenia caused by heparin and mediated by an atypical immune mechanism leading to a paradoxical high thrombotic risk, associated with severe morbidity or death. The diagnosis of HIT combines a clinical scoring of pretest probability and laboratory testing. First-line routine tests are antigen binding assays detecting specific antibodies. The most sensitive of these tests have a high HIT-negative predictive value enabling HIT diagnosis to be ruled out when negative. However, HIT-positive predictive value is low, and a functional assay evaluating the pathogenicity of the antibodies should be performed to exclude false-positive results. In contrast to screening assays, functional assays are highly specific but technically challenging, and are thus performed in referral laboratories, where platelet activation is detected using radioactive serotonin (serotonin release assay, SRA) or visually (heparin-induced platelet activation, HIPA). Flow cytometry is a possible alternative. It is, however, currently not widely used, mostly because of the lack of standardization of the published assays. This article describes and discusses the standardization of a HIT flow cytometry assay (HIT-FCA) method, which subsequently led to the development and commercialization of a CE-marked assay (HIT Confirm®, Emosis, France) as a suitable rapid HIT functional test.
- Subjects :
- Functional assay
Medicine (miscellaneous)
030204 cardiovascular system & hematology
General Biochemistry, Genetics and Molecular Biology
Article
Flow cytometry
03 medical and health sciences
0302 clinical medicine
Immune system
Heparin-induced thrombocytopenia
heparin-induced thrombocytopenia diagnosis
Medicine
Platelet activation
lcsh:QH301-705.5
medicine.diagnostic_test
biology
business.industry
flow cytometry
HIT functional assay
Heparin
medicine.disease
Pre- and post-test probability
lcsh:Biology (General)
Immunology
biology.protein
Antibody
business
030215 immunology
medicine.drug
Subjects
Details
- Language :
- English
- ISSN :
- 22279059
- Volume :
- 9
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Biomedicines
- Accession number :
- edsair.doi.dedup.....e187d6a1a0134003b78cf50d4fb555ba