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Complete genome sequence of a novel bromovirus infecting elderberry (Sambucus nigra L.) in the Czech Republic
- Source :
- Archives of Virology, Archives of Virology, Springer Verlag, 2018, 163 (2), pp.567-570. ⟨10.1007/s00705-017-3629-1⟩
- Publication Year :
- 2018
- Publisher :
- HAL CCSD, 2018.
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Abstract
- UMR BFP - Equipe Virologie; International audience; The genus Bromovirus currently contains six species whose members have relatively narrow host ranges. In the present work, a new bromovirus infecting elderberry (Sambucus nigra L.) is reported. dsRNA was purified and sequenced by next-generation sequencing, and with minimal additional completion by Sanger sequencing, the full tripartite genome was obtained. RNA1 is 3241 nt long and contains ORF1 (1a protein), RNA2 is 2810 nt long and contains ORF2 (2a protein), and RNA3 is 2244 nt long and contains ORF3a (movement protein) and ORF3b (coat protein, CP), separated by an intercistronic poly(A) stretch. Proteins 1a and 2a showed highest sequence identity (69.9% and 69.4%) to the corresponding proteins of melandrium yellow fleck virus. The coat protein showed highest sequence identity (67.9%) to that of brome mosaic virus. The genome shows a typical bromovirus organisation comprising of all the conserved protein domains within the genus. Phylogenetic analysis supports the assignment of this virus as a new member of the genus Bromovirus, for which the name "sambucus virus S" (SVS) is proposed.
- Subjects :
- 0301 basic medicine
Molecular Sequence Data
Genome, Viral
Sambucus nigra
Genome
03 medical and health sciences
symbols.namesake
Open Reading Frames
Brome mosaic virus
Virology
Movement protein
Phylogeny
Czech Republic
Plant Diseases
Whole genome sequencing
Sanger sequencing
biology
Base Sequence
Sambucus
General Medicine
biology.organism_classification
Bromovirus
[SDV.BV.PEP]Life Sciences [q-bio]/Vegetal Biology/Phytopathology and phytopharmacy
030104 developmental biology
[SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology
symbols
RNA, Viral
Subjects
Details
- Language :
- English
- ISSN :
- 03048608 and 14328798
- Database :
- OpenAIRE
- Journal :
- Archives of Virology, Archives of Virology, Springer Verlag, 2018, 163 (2), pp.567-570. ⟨10.1007/s00705-017-3629-1⟩
- Accession number :
- edsair.doi.dedup.....e17845e095dd2ae1ebfbb5bf08863847