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Isolation and retrieval of circulating tumor cells using centrifugal forces
- Source :
- Scientific Reports
- Publication Year :
- 2012
- Publisher :
- Nature Publishing Group, 2012.
-
Abstract
- Presence and frequency of rare circulating tumor cells (CTCs) in bloodstreams of cancer patients are pivotal to early cancer detection and treatment monitoring. Here, we use a spiral microchannel with inherent centrifugal forces for continuous, size-based separation of CTCs from blood (Dean Flow Fractionation (DFF)) which facilitates easy coupling with conventional downstream biological assays. Device performance was optimized using cancer cell lines (> 85% recovery), followed by clinical validation with positive CTCs enumeration in all samples from patients with metastatic lung cancer (n = 20; 5–88 CTCs per mL). The presence of CD133+ cells, a phenotypic marker characteristic of stem-like behavior in lung cancer cells was also identified in the isolated subpopulation of CTCs. The spiral biochip identifies and addresses key challenges of the next generation CTCs isolation assay including antibody independent isolation, high sensitivity and throughput (3 mL/hr); and single-step retrieval of viable CTCs.<br />Singapore. National Research Foundation (Singapore MIT Alliance for Research and Technology's BioSystems and Micromechanics Inter-Disciplinary Research programme)<br />Singapore. National Medical Research Council (grant NMRC 1225/2009)
- Subjects :
- Lung Neoplasms
Centrifugation
Cell Separation
Article
Circulating tumor cell
Antigens, CD
Cell Line, Tumor
medicine
Humans
AC133 Antigen
Early Cancer Detection
Lung cancer
Biochip
Glycoproteins
Blood Cells
Multidisciplinary
biology
Cancer
Microfluidic Analytical Techniques
Neoplastic Cells, Circulating
medicine.disease
Molecular biology
MCF-7 Cells
biology.protein
Keratins
Leukocyte Common Antigens
Antibody
Cancer cell lines
Peptides
Treatment monitoring
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Scientific Reports
- Accession number :
- edsair.doi.dedup.....e167fe87e7372f7db32be64c42869590