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Increased resistance to oxidative DNA damage of trabecular meshwork cells byE. coli FPGgene transfection
- Source :
- Free Radical Research. 45:751-758
- Publication Year :
- 2011
- Publisher :
- Informa UK Limited, 2011.
-
Abstract
- Oxidative damage plays a pathogenic role in various chronic degenerative diseases. Oxidative damage targeting trabecular meshwork (TM) cells as a consequence of mitochondrial damage is a pathogenic mechanism for glaucoma, the most common cause of irreversible blindness worldwide. Consequences of oxidative damage are attenuated by endocellular activities involved in scavenging reactive oxidative species and DNA repair. Selected bacterial genes are highly efficient at protecting cells from oxidative DNA damage. This situation occurs for Escherichia coli formamidopyrimidine DNA glycosylase (FPG), a major DNA glycosylase that repairs oxidatively damaged DNA. Accordingly, this study was aimed at transfecting human TM cells (HTMC) with Fpg in order to increase their resistance to oxidative damage. This study demonstrates that it is feasible to increase resistance of HTMC to endogenous oxidative damage by gene transfection. These findings bear relevance for primary and secondary prevention of degenerative glaucomas and other degenerative diseases where oxidative damage plays a pathogenic role.
- Subjects :
- DNA repair
Recombinant Fusion Proteins
genetics/metabolism
Gene Expression
biosynthesis/genetics
Biology
Transfection
medicine.disease_cause
Biochemistry
Cell Line
chemistry.chemical_compound
Trabecular Meshwork
Cell Line, Tumor
Escherichia coli
medicine
Humans
genetics
analogs /&/ derivatives/analysis
Tumor, DNA Damage, DNA-Formamidopyrimidine Glycosylase
genetics, Deoxyguanosine
analogs /&/ derivatives/analysis, Endothelial Cells
metabolism, Escherichia coli Proteins
genetics, Escherichia coli
genetics/metabolism, Gene Expression, Gene Therapy, Glaucoma
genetics/prevention /&/ control/therapy, Humans, Mitochondria
metabolism, Oxidation-Reduction, Oxidative Stress
genetics, Reactive Oxygen Species
metabolism, Recombinant Fusion Proteins
biosynthesis/genetics, Trabecular Meshwork
cytology/metabolism, Transfection
cytology/metabolism
chemistry.chemical_classification
Reactive oxygen species
Tumor
Escherichia coli Proteins
genetics/prevention /&/ control/therapy
Deoxyguanosine
Endothelial Cells
Glaucoma
Gene Therapy
Genetic Therapy
General Medicine
Formamidopyrimidine DNA glycosylase
Molecular biology
Mitochondria
Oxidative Stress
medicine.anatomical_structure
DNA-Formamidopyrimidine Glycosylase
chemistry
8-Hydroxy-2'-Deoxyguanosine
DNA glycosylase
Trabecular meshwork
Reactive Oxygen Species
metabolism
Oxidation-Reduction
DNA
DNA Damage
Subjects
Details
- ISSN :
- 10292470 and 10715762
- Volume :
- 45
- Database :
- OpenAIRE
- Journal :
- Free Radical Research
- Accession number :
- edsair.doi.dedup.....e16755a9e215be732a71e5df3274d074