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Sphere formation permits Oct4 reprogramming of ciliary body epithelial cells into induced pluripotent stem cells
- Source :
- Stem cells and development. 23(24)
- Publication Year :
- 2014
-
Abstract
- Somatic cells can be reprogrammed to induced pluripotent stem (iPS) cells by defined sets of transcription factors. We previously described reprogramming of monolayer-cultured adult mouse ciliary body epithelial (CE) cells by Oct4 and Klf4, but not with Oct4 alone. In this study, we report that Oct4 alone is sufficient to reprogram CE cells to iPS cells through sphere formation. Furthermore, we demonstrate that sphere formation induces a partial reprogramming state characterized by expression of retinal progenitor markers, upregulation of reprogramming transcription factors, such as Sall4 and Nanog, demethylation in the promoter regions of pluripotency associated genes, and mesenchymal to epithelial transition. The Oct4-iPS cells maintained normal karyotypes, expressed markers for pluripotent stem cells, and were capable of differentiating into derivatives of all three embryonic germ layers in vivo and in vitro. These findings suggest that sphere formation may render somatic cells more susceptible to reprogramming.
- Subjects :
- Homeobox protein NANOG
Induced stem cells
Ciliary Body
Induced Pluripotent Stem Cells
Kruppel-Like Transcription Factors
Epithelial Cells
Cell Biology
Hematology
Embryoid body
Biology
Cellular Reprogramming
Molecular biology
Endothelial stem cell
Kruppel-Like Factor 4
Mice
Original Research Reports
KLF4
Spheroids, Cellular
embryonic structures
Animals
Stem cell
Induced pluripotent stem cell
Reprogramming
Octamer Transcription Factor-3
Developmental Biology
Subjects
Details
- ISSN :
- 15578534
- Volume :
- 23
- Issue :
- 24
- Database :
- OpenAIRE
- Journal :
- Stem cells and development
- Accession number :
- edsair.doi.dedup.....e14839f6010ca73d599302811ebb21c2