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Antioxidant properties of proanthocyanidins attenuate carbon tetrachloride (CCl4)-induced steatosis and liver injury in rats via CYP2E1 regulation
- Source :
- Journal of medicinal food. 17(6)
- Publication Year :
- 2014
-
Abstract
- Liver steatosis is characterized by lipid dysregulation and fat accumulation in the liver and can lead to oxidative stress in liver. Since proanthocyanidins are present in plant-based foods and have powerful antioxidant properties, we investigated whether proanthocyanidins can prevent oxidative stress and subsequent liver injury. Carbon tetrachloride (CCl4) treatment can cause steatosis in rats that models both alcoholic and non-alcoholic fatty liver disease in humans. We pre-treated rats by oral administration of proanthocyanidins extracted from grape seeds 7 days prior to intragastrically administering CCl4. Proanthocyanidin treatment continued for an additional 2 weeks, after which time liver and serum were harvested, and mediators of liver injury, oxidative stress, and histological features were evaluated. CCl4-treated rats exhibited significant increases in the following parameters as compared to non-treated rats: fat droplets in the liver, liver injury (ALT, AST), and DNA damage (8-OHdG). Additionally, CCl4 treatment decreased antioxidant enzymes SOD, GSH, GPX, and CAT in the liver due to their rapid depletion after battling against oxidative stress. Compared to CCl4-treated rats, treatment with proanthocyanidins effectively suppressed lipid accumulation, liver injury, DNA damage, as well as restored antioxidant enzyme levels. Further investigation revealed that proanthocyanidins treatment also inhibited expression of CYP2E1 in liver, which prevented the initial step of generating free radicals from CCl4. The data presented here show that treatment with orally administered proanthocyanidins prevented liver injury in the CCl4-induced steatosis model, likely through exerting antioxidant actions to suppress oxidative stress and inhibiting the free radical–generating CYP2E1 enzyme.
- Subjects :
- Male
Antioxidant
medicine.medical_treatment
Medicine (miscellaneous)
CCL4
Pharmacology
medicine.disease_cause
digestive system
Antioxidants
chemistry.chemical_compound
medicine
Animals
Proanthocyanidins
Vitis
Rats, Wistar
Carbon Tetrachloride
Liver injury
Nutrition and Dietetics
Chemistry
Plant Extracts
Fatty liver
food and beverages
Cytochrome P-450 CYP2E1
CYP2E1
medicine.disease
Fatty Liver
Oxidative Stress
Biochemistry
Liver
Seeds
Carbon tetrachloride
Steatosis
Chemical and Drug Induced Liver Injury
Full Communications
Oxidative stress
Phytotherapy
Subjects
Details
- ISSN :
- 15577600
- Volume :
- 17
- Issue :
- 6
- Database :
- OpenAIRE
- Journal :
- Journal of medicinal food
- Accession number :
- edsair.doi.dedup.....e119f633c03ca53ed7dbb2bef454e189