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An autocrine role for endothelin-1 in the regulation of proximal tubule NHE3
- Source :
- Kidney International. 65:1320-1326
- Publication Year :
- 2004
- Publisher :
- Elsevier BV, 2004.
-
Abstract
- An autocrine role for endothelin-1 in the regulation of proximal tubule NHE3. Background Chronic metabolic acidosis leads to an increase in NHE3 activity that is mediated by endothelin-1 (ET-1) expression and activation of the proximal tubule endothelin B receptor. Chronic metabolic acidosis increases preproET-1 mRNA abundance in kidney cortex, but the cell responsible has not been identified. Methods PreproET-1 mRNA abundance was quantified by competitive reverse transcription-polymerase chain reaction (RT-PCR) on tissue harvested from control rats or rats in which chronic metabolic acidosis was induced by addition of NH 4 Cl to the drinking water. Results Chronic metabolic acidosis leads to an increase in preproET-1 mRNA expression in kidney cortex, proximal tubules, and glomeruli. The increase in preproET-1 expression correlates with the decrease in blood [HCO 3 − ]. ET-1 expression is also increased by acidosis in abdominal aorta, but not in cardiac muscle. Conclusion In the renal proximal tubule, chronic metabolic acidosis induces an increase in preproET-1 expression, providing a mechanism for autocrine regulation of proximal tubule NHE3 activity. This response is not unique to the proximal tubule cell, but is also not ubiquitous.
- Subjects :
- Male
chronic metabolic acidosis
medicine.medical_specialty
Kidney Cortex
Sodium-Hydrogen Exchangers
Kidney Glomerulus
Cell
heart
In Vitro Techniques
Kidney Tubules, Proximal
Rats, Sprague-Dawley
Internal medicine
medicine
Animals
RNA, Messenger
Autocrine signalling
Na+/H+ antiporter
Acidosis
Messenger RNA
Endothelin-1
Sodium-Hydrogen Exchanger 3
urogenital system
Chemistry
Myocardium
Chronic metabolic acidosis
Cardiac muscle
Metabolic acidosis
medicine.disease
autocrine regulation
Endothelin 1
Rats
Autocrine Communication
aorta
Endocrinology
medicine.anatomical_structure
Nephrology
Chronic Disease
medicine.symptom
Subjects
Details
- ISSN :
- 00852538
- Volume :
- 65
- Database :
- OpenAIRE
- Journal :
- Kidney International
- Accession number :
- edsair.doi.dedup.....e116b6b9d93ef39b8843918556f6f4e0